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Yorodumi- PDB-2l33: Solution NMR Structure of DRBM 2 domain of Interleukin enhancer-b... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2l33 | ||||||
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Title | Solution NMR Structure of DRBM 2 domain of Interleukin enhancer-binding factor 3 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4527E | ||||||
Components | Interleukin enhancer-binding factor 3 | ||||||
Keywords | Transcription regulator / Structural Genomics / NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG) / PSI-Biology / Protein Structure Initiative / DRBM / dsRNA-binding / ILF3 / NF90 | ||||||
Function / homology | Function and homology information Regulation of CDH11 gene transcription / mRNA 3'-UTR AU-rich region binding / negative regulation of viral genome replication / PKR-mediated signaling / double-stranded RNA binding / virus receptor activity / defense response to virus / negative regulation of translation / single-stranded RNA binding / ribonucleoprotein complex ...Regulation of CDH11 gene transcription / mRNA 3'-UTR AU-rich region binding / negative regulation of viral genome replication / PKR-mediated signaling / double-stranded RNA binding / virus receptor activity / defense response to virus / negative regulation of translation / single-stranded RNA binding / ribonucleoprotein complex / protein phosphorylation / negative regulation of DNA-templated transcription / nucleolus / positive regulation of DNA-templated transcription / mitochondrion / DNA binding / RNA binding / extracellular region / nucleoplasm / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / distance geometry, molecular dynamics, torsion angle dynamics, simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Liu, G. / Janjua, H. / Xiao, R. / Acton, T.B. / Ciccosanti, A. / Shastry, R.B. / Everett, J. / Montelione, G.T. / Northeast Structural Genomics Consortium / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: To be Published Title: Northeast Structural Genomics Consortium Target HR4527E Authors: Liu, G. / Janjua, H. / Xiao, R. / Acton, T.B. / Everett, J. / Montelione, G.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2l33.cif.gz | 630.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2l33.ent.gz | 540.1 KB | Display | PDB format |
PDBx/mmJSON format | 2l33.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l3/2l33 ftp://data.pdbj.org/pub/pdb/validation_reports/l3/2l33 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10189.675 Da / Num. of mol.: 1 / Fragment: DRBM 2 domain residues 521-600 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ILF3, DRBF, MPHOSPH4, NF90 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)+ Magic / References: UniProt: Q12906 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: double-stranded RNA-binding domains DRBM2 | ||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry, molecular dynamics, torsion angle dynamics, simulated annealing Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |