- PDB-2kz3: Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Ra... -
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Basic information
Entry
Database: PDB / ID: 2kz3
Title
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Rad51D from 1 to 83
Components
Putative uncharacterized protein RAD51L3
Keywords
UNKNOWN FUNCTION / Rad51D / Homologous Recombination
Function / homology
Function and homology information
Rad51B-Rad51C-Rad51D-XRCC2 complex / telomere maintenance via recombination / Impaired BRCA2 binding to PALB2 / DNA strand invasion / gamma-tubulin binding / reciprocal meiotic recombination / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange ...Rad51B-Rad51C-Rad51D-XRCC2 complex / telomere maintenance via recombination / Impaired BRCA2 binding to PALB2 / DNA strand invasion / gamma-tubulin binding / reciprocal meiotic recombination / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / ATP-dependent DNA damage sensor activity / Presynaptic phase of homologous DNA pairing and strand exchange / ATP-dependent activity, acting on DNA / interstrand cross-link repair / four-way junction DNA binding / telomere maintenance / replication fork / TP53 Regulates Transcription of DNA Repair Genes / double-strand break repair via homologous recombination / HDR through Homologous Recombination (HRR) / single-stranded DNA binding / chromosome, telomeric region / regulation of cell cycle / DNA repair / centrosome / ATP hydrolysis activity / DNA binding / nucleoplasm / ATP binding / nucleus / cytoplasm Similarity search - Function
: / : / RAD51D, N-terminal domain / DNA recombination and repair protein, RecA-like / DNA recombination and repair protein Rad51-like, C-terminal / Rad51 / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Mass: 9070.666 Da / Num. of mol.: 1 / Fragment: UNP residues 1-83 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) / References: UniProt: C9JSQ7, UniProt: O75771*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
3
2D 1H-15N HSQC
1
2
3
3D 1H-15N NOESY
1
3
3
3D 1H-15N TOCSY
1
4
2
3D (H)CCH-TOCSY
1
5
2
3D 1H-13C NOESY
1
6
1
3DCBCA(CO)NH
1
7
1
3D HN(CA)CB
1
8
1
3D HNCO
1
9
1
3DH(CCO)NH
1
10
1
3DC(CO)NH
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
1 mM [U-100% 15N] human Rad51D_N-1, 1 mM [U-13C; U-15N] human Rad51D_N-2, 1 mM DTT-3, 0.02 % sodium azide-4, 1 mM [U-99% 13C; U-99% 15N] human Rad51D_N-5, 100% D2O
100% D2O
2
1 mM [U-99% 13C; U-99% 15N] human Rad51D_N-6, 25 mM TRIS-7, 100 mM sodium chloride-8, 1 mM DTT-9, 0.02 % sodium azide-10, 100% D2O
100% D2O
3
1 mM [U-99% 15N] human Rad51D_N-11, 25 mM TRIS-12, 100 mM sodium chloride-13, 1 mM DTT-14, 0.02 % sodium azide-15, 90% H2O/10% D2O
Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1
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