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基本情報
登録情報 | データベース: PDB / ID: 2kyv | ||||||
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タイトル | Hybrid solution and solid-state NMR structure ensemble of phospholamban pentamer | ||||||
![]() | Phospholamban | ||||||
![]() | MEMBRANE PROTEIN / phospholamban / solid state NMR / hybrid method | ||||||
機能・相同性 | ![]() negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of relaxation of muscle / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / negative regulation of calcium ion transmembrane transporter activity / acrosome assembly / negative regulation of calcium ion import ...negative regulation of calcium ion binding / negative regulation of calcium ion import into sarcoplasmic reticulum / negative regulation of ATPase-coupled calcium transmembrane transporter activity / adenylate cyclase-activating adrenergic receptor signaling pathway involved in heart process / regulation of relaxation of muscle / regulation of the force of heart contraction by cardiac conduction / calcium ion-transporting ATPase complex / negative regulation of calcium ion transmembrane transporter activity / acrosome assembly / negative regulation of calcium ion import / negative regulation of catalytic activity / ATPase inhibitor activity / cardiac muscle tissue development / regulation of cardiac muscle cell contraction / enzyme inhibitor activity / negative regulation of heart rate / muscle cell cellular homeostasis / regulation of calcium ion transport / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / Notch signaling pathway / sarcoplasmic reticulum membrane / mitochondrial membrane / intracellular calcium ion homeostasis / ATPase binding / endoplasmic reticulum membrane / perinuclear region of cytoplasm / endoplasmic reticulum / protein homodimerization activity / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 溶液NMR / 個体NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Verardi, R. / Shi, L. / Traaseth, N.J. / Veglia, G. | ||||||
![]() | ![]() タイトル: Structural topology of phospholamban pentamer in lipid bilayers by a hybrid solution and solid-state NMR method. 著者: Verardi, R. / Shi, L. / Traaseth, N.J. / Walsh, N. / Veglia, G. | ||||||
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構造ビューア | 分子: ![]() ![]() |
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文書・要旨 | ![]() | 360.2 KB | 表示 | ![]() |
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登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 6099.499 Da / 分子数: 5 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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-実験情報
-実験
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NMR実験 |
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NMRスペクトロメーター |
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解析
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 2155 / NOE intraresidue total count: 639 / NOE long range total count: 85 / NOE medium range total count: 656 / NOE sequential total count: 775 / Protein chi angle constraints total count: 127 / Protein other angle constraints total count: 65 / Protein phi angle constraints total count: 140 / Protein psi angle constraints total count: 145 | ||||||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with acceptable covalent geometry 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 / Maximum lower distance constraint violation: 0.5 Å / Maximum torsion angle constraint violation: 0.7 ° / Maximum upper distance constraint violation: 0.5 Å / Torsion angle constraint violation method: xplor-nih | ||||||||||||||||||||||||||||||||
NMR ensemble rms | Distance rms dev: 0.05 Å / Distance rms dev error: 0.003 Å |