+Open data
-Basic information
Entry | Database: PDB / ID: 2kg2 | ||||||
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Title | Solution structure of a PDZ protein | ||||||
Components | Tax1-binding protein 3 | ||||||
Keywords | PROTEIN BINDING / PDZ domain / Cytoplasm / Nucleus / Phosphoprotein / Wnt signaling pathway | ||||||
Function / homology | Function and homology information negative regulation of protein localization to cell surface / RHO GTPases Activate Rhotekin and Rhophilins / activation of GTPase activity / negative regulation of Wnt signaling pathway / Rho protein signal transduction / fibrillar center / beta-catenin binding / Wnt signaling pathway / actin cytoskeleton / negative regulation of cell population proliferation ...negative regulation of protein localization to cell surface / RHO GTPases Activate Rhotekin and Rhophilins / activation of GTPase activity / negative regulation of Wnt signaling pathway / Rho protein signal transduction / fibrillar center / beta-catenin binding / Wnt signaling pathway / actin cytoskeleton / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / extracellular exosome / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Model details | lowest energy, model 2 | ||||||
Authors | Durney, M.A. / Birrane, G. / Anklin, C. / Soni, A. / Ladias, J.A.A. | ||||||
Citation | Journal: J.Biomol.Nmr / Year: 2009 Title: Solution structure of the human Tax-interacting protein-1. Authors: Durney, M.A. / Birrane, G. / Anklin, C. / Soni, A. / Ladias, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kg2.cif.gz | 746.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kg2.ent.gz | 647.4 KB | Display | PDB format |
PDBx/mmJSON format | 2kg2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kg/2kg2 ftp://data.pdbj.org/pub/pdb/validation_reports/kg/2kg2 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13677.541 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TAX1BP3, TIP1 / Plasmid: pHMTH / Production host: Escherichia coli (E. coli) / References: UniProt: O14907 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1-2 mM [U-100% 13C; U-100% 15N] TIP-1, 93% H2O/7% D2O Solvent system: 93% H2O/7% D2O |
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Sample | Units: mM / Component: TIP-1 / Isotopic labeling: [U-100% 13C; U-100% 15N] / Conc. range: 1-2 |
Sample conditions | Ionic strength: 50 / pH: 6.5 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software | Name: CNS / Developer: Brunger A. T. et.al. / Classification: refinement |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 |
NMR representative | Selection criteria: lowest energy |
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |