+Open data
-Basic information
Entry | Database: PDB / ID: 2kb2 | ||||||
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Title | BlrP1 BLUF | ||||||
Components | BlrP1 | ||||||
Keywords | SIGNALING PROTEIN / HYDROLASE REGULATOR / BLUF / Photoreceptor / HYDROLASE | ||||||
Function / homology | Function and homology information blue light photoreceptor activity / FAD binding / identical protein binding / metal ion binding Similarity search - Function | ||||||
Biological species | Klebsiella pneumoniae subsp. pneumoniae MGH 78578 (bacteria) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Wu, Q. / Gardner, K.H. | ||||||
Citation | Journal: Biochemistry / Year: 2009 Title: Structure and insight into blue light-induced changes in the BlrP1 BLUF domain Authors: Wu, Q. / Gardner, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kb2.cif.gz | 947.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kb2.ent.gz | 785.8 KB | Display | PDB format |
PDBx/mmJSON format | 2kb2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kb2_validation.pdf.gz | 504.4 KB | Display | wwPDB validaton report |
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Full document | 2kb2_full_validation.pdf.gz | 660 KB | Display | |
Data in XML | 2kb2_validation.xml.gz | 86.1 KB | Display | |
Data in CIF | 2kb2_validation.cif.gz | 99.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kb/2kb2 ftp://data.pdbj.org/pub/pdb/validation_reports/kb/2kb2 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16924.289 Da / Num. of mol.: 1 / Fragment: BLUF Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Klebsiella pneumoniae subsp. pneumoniae MGH 78578 (bacteria) Species: pneumoniae / Gene: KPN78578_15680, KPN_01598 / Production host: Escherichia coli (E. coli) / References: UniProt: A6T8V8 |
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#2: Chemical | ChemComp-FMN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.1-0.7 mM [U-99% 13C; U-99% 15N] BlrP1 BLUF, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.1 mM / Component: BlrP1 BLUF / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Ionic strength: 30 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 500 / Conformers submitted total number: 20 |