[English] 日本語
Yorodumi- PDB-2k4v: Solution structure of uncharacterized protein PA1076 from Pseudom... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2k4v | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of uncharacterized protein PA1076 from Pseudomonas aeruginosa. Northeast Structural Genomics Consortium (NESG) target PaT3, Ontario Center for Structural Proteomics target PA1076 . | ||||||
Components | uncharacterized protein PA1076 | ||||||
Keywords | structural genomics / unknown function / PSI-2 / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG / Ontario Centre for Structural Proteomics / OCSP | ||||||
Function / homology | Domain of unknown function DUF5064 / Protein of unknown function DUF5064 / Domain of unknown function (DUF5064) / Double Stranded RNA Binding Domain / 2-Layer Sandwich / Alpha Beta / Uncharacterized protein Function and homology information | ||||||
Biological species | Pseudomonas aeruginosa (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Gutmanas, A. / Lemak, A. / Fares, C. / Yee, A. / Semesi, A. / Arrowsmith, C.H. / Montelione, G.T. / Northeast Structural Genomics Consortium (NESG) / Ontario Centre for Structural Proteomics (OCSP) | ||||||
Citation | Journal: To be Published Title: Solution structure of uncharacterized protein PA1076 from Pseudomonas aeruginosa. Authors: Gutmanas, A. / Lemak, A. / Fares, C. / Yee, A. / Semesi, A. / Arrowsmith, C.H. #1: Journal: J.Biomol.Nmr / Year: 2011 Title: A novel strategy for NMR resonance assignment and protein structure determination. Authors: Lemak, A. / Gutmanas, A. / Chitayat, S. / Karra, M. / Fares, C. / Sunnerhagen, M. / Arrowsmith, C.H. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2k4v.cif.gz | 762.7 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2k4v.ent.gz | 641 KB | Display | PDB format |
PDBx/mmJSON format | 2k4v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k4/2k4v ftp://data.pdbj.org/pub/pdb/validation_reports/k4/2k4v | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 14233.822 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) / Species: aeruginosa / Gene: PA1076 / Plasmid: p11 / Species (production host): coli / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 DE3 / Variant (production host): star magic / References: UniProt: Q9I4Q3 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
NMR details | Text: 5 mm Shigemi tube Most 3D spectra collected using non-uniform sampling and processed with MDDNMR |
-Sample preparation
Details | Contents: 0.7-1 mM [U-100% 13C; U-100% 15N] PA1076, 1x inhibitor cocktail (purchased from Roche), 10 mM [U-2H] TRIS, 300 mM sodium chloride, 0.01 % sodium azide, 1 mM benzamidine, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||||||||
Sample conditions | Ionic strength: 300 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 1 / Details: water refinement with RDC constraints | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |