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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2jp1 | ||||||
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タイトル | Solution structure of the alternative conformation of XCL1/Lymphotactin | ||||||
![]() | Lymphotactin | ||||||
![]() | CYTOKINE / Lymphotactin / XCL1 / chemokine / structural rearrangement / protein folding | ||||||
機能・相同性 | ![]() mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis ...mature natural killer cell chemotaxis / positive regulation of granzyme A production / negative regulation of T-helper 1 cell activation / positive regulation of immunoglobulin production in mucosal tissue / positive regulation of thymocyte migration / positive regulation of granzyme B production / negative regulation of T-helper 1 type immune response / positive regulation of B cell chemotaxis / negative regulation of T cell cytokine production / positive regulation of natural killer cell chemotaxis / chemokine receptor binding / positive regulation of T-helper 1 cell cytokine production / positive regulation of transforming growth factor beta production / positive regulation of T cell chemotaxis / positive regulation of T-helper 2 cell cytokine production / CCR chemokine receptor binding / lymphocyte chemotaxis / positive regulation of CD4-positive, alpha-beta T cell proliferation / negative regulation of CD4-positive, alpha-beta T cell proliferation / eosinophil chemotaxis / chemokine-mediated signaling pathway / positive regulation of neutrophil chemotaxis / Chemokine receptors bind chemokines / chemokine activity / positive regulation of leukocyte chemotaxis / negative regulation of interleukin-2 production / positive regulation of CD8-positive, alpha-beta T cell proliferation / monocyte chemotaxis / positive regulation of interleukin-10 production / negative regulation of type II interferon production / cellular response to interleukin-1 / cellular response to interleukin-4 / release of sequestered calcium ion into cytosol / cellular response to transforming growth factor beta stimulus / neutrophil chemotaxis / positive regulation of release of sequestered calcium ion into cytosol / response to virus / negative regulation of DNA-binding transcription factor activity / positive regulation of T cell cytokine production / cellular response to type II interferon / positive regulation of T cell mediated cytotoxicity / cell-cell signaling / cellular response to tumor necrosis factor / regulation of inflammatory response / G alpha (q) signalling events / positive regulation of ERK1 and ERK2 cascade / inflammatory response / G protein-coupled receptor signaling pathway / negative regulation of DNA-templated transcription / signal transduction / protein homodimerization activity / extracellular space / extracellular region 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | 溶液NMR / AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT, ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENT | ||||||
![]() | Volkman, B.F. / Tuinstra, R.L. / Peterson, F.C. | ||||||
![]() | ![]() タイトル: Interconversion between two unrelated protein folds in the lymphotactin native state 著者: Tuinstra, R.L. / Peterson, F.C. / Kutlesa, S. / Elgin, E.S. / Kron, M.A. / Volkman, B.F. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 834 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 365.4 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 631.7 KB | 表示 | |
XML形式データ | ![]() | 34.6 KB | 表示 | |
CIF形式データ | ![]() | 61.8 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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NMR アンサンブル |
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要素
#1: タンパク質 | 分子量: 10286.789 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||
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NMR実験 |
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試料調製
詳細 |
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試料 |
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試料状態 | イオン強度: 22 / pH: 6.0 / 圧: AMBIENT / 温度: 313 K |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Bruker DRX / 製造業者: Bruker / モデル: DRX / 磁場強度: 600 MHz |
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解析
NMR software |
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精密化 | 手法: AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT, ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENT ソフトェア番号: 1 詳細: HOMODIMER STRUCTURES ARE BASED ON A TOTAL OF 1295 NOE CONSTRAINTS ( 320 INTRA, 370 SEQUENTIAL, 104 MEDIUM, 420 LONG RANGE and 81 INTERMONOMER CONSTRAINTS) AND 131 PHI AND PSI DIHEDRAL ANGLE ...詳細: HOMODIMER STRUCTURES ARE BASED ON A TOTAL OF 1295 NOE CONSTRAINTS ( 320 INTRA, 370 SEQUENTIAL, 104 MEDIUM, 420 LONG RANGE and 81 INTERMONOMER CONSTRAINTS) AND 131 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS. CONSTRAINT WERE IN ONE ASSIGNED AND VALIDATED IN ONE MONOMER AND THEN DUPLICATED TO GENERATE A SYMMETRY RELATED CONSTRAINT IN THE SECOND MONOMER. CONSTRAINT TOTALS LISTED ABOVE INCLUDE CONSTRAINTS FROM BOTH MONOMERS. | ||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 1295 / NOE intraresidue total count: 320 / NOE long range total count: 420 / NOE medium range total count: 104 / NOE sequential total count: 370 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 64 / Protein psi angle constraints total count: 65 | ||||||||||||||||||||||||||||||||
代表構造 | 選択基準: lowest energy | ||||||||||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: target function / 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |