+Open data
-Basic information
Entry | Database: PDB / ID: 2jkf | ||||||
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Title | Plasmodium falciparum profilin | ||||||
Components | PROFILIN | ||||||
Keywords | PROTEIN BINDING / PROLINE-RICH LIGAND / PLASMODIUM FALCIPARUM / MALARIA / PROFILIN / CYTOSKELETON / ACTIN FILAMENT STRUCTURAL PROTEIN / PROTEIN-BINDING | ||||||
Function / homology | Function and homology information cytoplasmic actin-based contraction involved in cell motility / sequestering of actin monomers / actin monomer binding / phospholipid binding / actin cytoskeleton / actin binding / cell cortex / actin cytoskeleton organization / cytoplasm Similarity search - Function | ||||||
Biological species | PLASMODIUM FALCIPARUM (malaria parasite P. falciparum) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.31 Å | ||||||
Authors | Kursula, I. / Kursula, P. / Ganter, M. / Panjikar, S. / Matuschewski, K. / Schueler, H. | ||||||
Citation | Journal: Structure / Year: 2008 Title: Structural Basis for Parasite-Specific Functions of the Divergent Profilin of Plasmodium Falciparum Authors: Kursula, I. / Kursula, P. / Ganter, M. / Panjikar, S. / Matuschewski, K. / Schueler, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2jkf.cif.gz | 47.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2jkf.ent.gz | 34.5 KB | Display | PDB format |
PDBx/mmJSON format | 2jkf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2jkf_validation.pdf.gz | 427.6 KB | Display | wwPDB validaton report |
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Full document | 2jkf_full_validation.pdf.gz | 431 KB | Display | |
Data in XML | 2jkf_validation.xml.gz | 9.6 KB | Display | |
Data in CIF | 2jkf_validation.cif.gz | 12.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/jk/2jkf ftp://data.pdbj.org/pub/pdb/validation_reports/jk/2jkf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 20102.010 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PLASMODIUM FALCIPARUM (malaria parasite P. falciparum) Description: CODONPLUSRIPL / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q8I2J4, UniProt: P86294*PLUS |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50 % / Description: NONE |
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Crystal grow | pH: 6 / Details: 2.2 M AMMONIUM SULFATE, PH 6 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: MAX II / Beamline: I911-3 / Wavelength: 1 |
Detector | Type: MARRESEARCH / Detector: CCD / Details: RH-COATED SI MIRRORS |
Radiation | Monochromator: SI(111) DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.31→20 Å / Num. obs: 8883 / % possible obs: 96.4 % / Observed criterion σ(I): -3 / Redundancy: 6.4 % / Biso Wilson estimate: 36 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 25 |
Reflection shell | Resolution: 2.31→2.37 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.2 / Mean I/σ(I) obs: 6.8 / % possible all: 85 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.31→20 Å / Cor.coef. Fo:Fc: 0.933 / Cor.coef. Fo:Fc free: 0.897 / SU B: 17.058 / SU ML: 0.19 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.341 / ESU R Free: 0.262 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES RESIDUAL ONLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.45 Å2
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Refinement step | Cycle: LAST / Resolution: 2.31→20 Å
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Refine LS restraints |
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