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- PDB-2j48: NMR structure of the pseudo-receiver domain of the CikA protein. -

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Basic information

Entry
Database: PDB / ID: 2j48
TitleNMR structure of the pseudo-receiver domain of the CikA protein.
ComponentsTWO-COMPONENT SENSOR KINASE
KeywordsTRANSFERASE / KINASE / PSEUDO-RECEIVER / CIRCADIAN CLOCK / RESPONSE REGULATOR / HISTIDINE PROTEIN KINASE
Function / homology
Function and homology information


Transferases; Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor / : / phosphorelay sensor kinase activity / ATP binding
Similarity search - Function
Phytochrome chromophore attachment domain / Phytochrome chromophore attachment site domain profile. / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / cheY-homologous receiver domain ...Phytochrome chromophore attachment domain / Phytochrome chromophore attachment site domain profile. / His Kinase A (phospho-acceptor) domain / His Kinase A (phosphoacceptor) domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain / Signal transduction histidine kinase, dimerisation/phosphoacceptor domain superfamily / Signal transduction histidine kinase-related protein, C-terminal / Histidine kinase domain / Histidine kinase domain profile. / cheY-homologous receiver domain / Signal transduction response regulator, receiver domain / Response regulatory domain profile. / GAF domain / CheY-like superfamily / Domain present in phytochromes and cGMP-specific phosphodiesterases. / GAF domain / GAF-like domain superfamily / Response regulator / Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase / Histidine kinase-like ATPases / Histidine kinase/HSP90-like ATPase / Histidine kinase/HSP90-like ATPase superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
Two-component sensor kinase / Circadian input kinase
Similarity search - Component
Biological speciesSYNECHOCOCCUS ELONGATUS (bacteria)
MethodSOLUTION NMR / XPLOR-NIH
AuthorsGao, T. / Zhang, X. / Golden, S.S. / LiWang, A.
CitationJournal: Protein Sci. / Year: 2007
Title: NMR structure of the pseudo-receiver domain of CikA.
Authors: Gao, T. / Zhang, X. / Ivleva, N.B. / Golden, S.S. / LiWang, A.
History
DepositionAug 26, 2006Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 6, 2007Provider: repository / Type: Initial release
Revision 1.1May 8, 2011Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 28, 2018Group: Database references / Source and taxonomy / Category: citation / citation_author / entity_src_gen
Item: _citation.page_last / _citation.pdbx_database_id_DOI ..._citation.page_last / _citation.pdbx_database_id_DOI / _citation.title / _citation_author.name / _entity_src_gen.pdbx_host_org_ncbi_taxonomy_id / _entity_src_gen.pdbx_host_org_scientific_name / _entity_src_gen.pdbx_host_org_strain / _entity_src_gen.pdbx_host_org_variant
Revision 1.4May 15, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_nmr_software
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_mr / _pdbx_nmr_software.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: TWO-COMPONENT SENSOR KINASE


Theoretical massNumber of molelcules
Total (without water)12,8851
Polymers12,8851
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100ENERGY
Representative

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Components

#1: Protein TWO-COMPONENT SENSOR KINASE


Mass: 12884.989 Da / Num. of mol.: 1 / Fragment: PSEUDO-RECEIVER DOMAIN, RESIDUES 627-745
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) SYNECHOCOCCUS ELONGATUS (bacteria) / Strain: PCC 7942 / Plasmid: PET-15B / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q5N3P8, UniProt: Q9KHI5*PLUS, histidine kinase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111NOESY-TYPE
121COSY-TYPE
NMR detailsText: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON THE 13C, 15N-LABELED PSEUDO-RECEIVER DOMAIN OF CIKA

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Sample preparation

DetailsContents: 95% H2O/5% D2O
Sample conditionsIonic strength: 70 mM / pH: 7.0 / Pressure: 1.0 atm / Temperature: 298.0 K

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NMR measurement

NMR spectrometerType: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz

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Processing

NMR software
NameDeveloperClassification
Xplor-NIHBRUNGER,C.D.SCHWIETERS,J.J.KUSZEWSKI, G.M.CLORErefinement
Xplor-NIHstructure solution
NMRPipestructure solution
PIPPstructure solution
NMRDrawstructure solution
RefinementMethod: XPLOR-NIH / Software ordinal: 1
Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.
NMR ensembleConformer selection criteria: ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 20

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