The second part of the biological assembly is generated by the symmetry operation (-X, -Y, Z)
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Components
#1: Protein
ZincfingerandBTBdomain-containingprotein7A / Leukemia/lymphoma- related factor / Factor that binds to inducer of short transcripts protein 1 / ...Leukemia/lymphoma- related factor / Factor that binds to inducer of short transcripts protein 1 / Factor binding IST protein 1 / FBI-1 / HIV-1 1st-binding protein 1 / TTF-I-interacting peptide 21 / TIP21
Mass: 12939.632 Da / Num. of mol.: 1 / Fragment: BTB/POZ-domain, residues 9-128 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ZBTB7A, FBI1, LRF, ZBTB7 / Plasmid: pJT18 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O95365
Mass: 18.015 Da / Num. of mol.: 117 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION
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Sample preparation
Crystal
Density Matthews: 3.4 Å3/Da / Density % sol: 63.87 %
Crystal grow
Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein at 24 mg/ml in 25 mM Tris, 150 mM NaCl, 2mM DTT, 0.18 M Calcium acetate hydrate, 18% w/v Polyethylene glycol 3350, 0.01 M Praseodymium (III) Acetate, pH 7.5, VAPOR DIFFUSION, SITTING ...Details: Protein at 24 mg/ml in 25 mM Tris, 150 mM NaCl, 2mM DTT, 0.18 M Calcium acetate hydrate, 18% w/v Polyethylene glycol 3350, 0.01 M Praseodymium (III) Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K
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