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- PDB-2hrj: NMR solution structure of the F2 subdomain of talin -

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Basic information

Entry
Database: PDB / ID: 2hrj
TitleNMR solution structure of the F2 subdomain of talin
ComponentsTalin-1
KeywordsSTRUCTURAL PROTEIN / ACBP-like / talin
Function / homology
Function and homology information


ruffle / structural constituent of cytoskeleton / ruffle membrane / platelet aggregation / cell-cell adhesion / actin filament binding / integrin binding / cytoskeleton / focal adhesion / cell surface ...ruffle / structural constituent of cytoskeleton / ruffle membrane / platelet aggregation / cell-cell adhesion / actin filament binding / integrin binding / cytoskeleton / focal adhesion / cell surface / plasma membrane / cytosol
Similarity search - Function
: / Talin, R4 domain / Vinculin-binding site-containing domain / Talin, central / Talin, N-terminal F0 domain / Talin, central domain superfamily / Talin-1/2, rod-segment / Vinculin Binding Site / Talin, middle domain / N-terminal or F0 domain of Talin-head FERM ...: / Talin, R4 domain / Vinculin-binding site-containing domain / Talin, central / Talin, N-terminal F0 domain / Talin, central domain superfamily / Talin-1/2, rod-segment / Vinculin Binding Site / Talin, middle domain / N-terminal or F0 domain of Talin-head FERM / I/LWEQ domain / I/LWEQ domain superfamily / I/LWEQ domain / I/LWEQ domain profile. / I/LWEQ domain / Acyl-CoA Binding Protein - #10 / Phosphotyrosine-binding domain / Acyl-CoA Binding Protein / Alpha-catenin/vinculin-like superfamily / FERM, N-terminal / FERM N-terminal domain / FERM domain signature 1. / FERM conserved site / FERM domain signature 2. / FERM central domain / FERM/acyl-CoA-binding protein superfamily / FERM central domain / FERM superfamily, second domain / FERM domain / FERM domain profile. / Band 4.1 domain / Band 4.1 homologues / PH-like domain superfamily / Ubiquitin-like domain superfamily / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
Biological speciesGallus gallus (chicken)
MethodSOLUTION NMR / simulated annealing, torsion angle dynamics
AuthorsLiu, G. / Arroyo, M.M. / Zheng, J.
CitationJournal: To be Published
Title: Structural characterization of the talin F2 FERM subdomain with Coenzyme A and PI(4,5)P2
Authors: Arroyo, M.M. / Liu, G. / Guibao, C.D. / Zheng, J.
History
DepositionJul 20, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0Apr 29, 2008Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2011Group: Version format compliance
Revision 1.2Mar 9, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_spectrometer ...database_2 / pdbx_nmr_spectrometer / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_spectrometer.model
Revision 1.3May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Talin-1


Theoretical massNumber of molelcules
Total (without water)14,1811
Polymers14,1811
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)25 / 200target function
RepresentativeModel #1lowest energy

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Components

#1: Protein Talin-1


Mass: 14181.270 Da / Num. of mol.: 1 / Fragment: F2 subdomain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TLN1, TLN / Production host: Escherichia coli (E. coli) / References: UniProt: P54939

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Sample conditions
Conditions-IDpHPressure (kPa)Temperature (K)
16.5 ambient 298 K
26.5 ambient 310 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE8001
Bruker AVANCEBrukerAVANCE6002
Varian INOVAVarianINOVA6003

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Processing

NMR software
NameDeveloperClassification
DIANAstructure solution
DIANArefinement
X-PLORBRUNGERdata analysis
NMRPipeprocessing
RefinementMethod: simulated annealing, torsion angle dynamics / Software ordinal: 1
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 25

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