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- PDB-2h0s: Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS r... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2h0s | ||||||
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Title | Pre-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme | ||||||
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![]() | RNA / Ribozyme / Pseudoknot / Helix | ||||||
Function / homology | : / DNA/RNA hybrid / DNA/RNA hybrid (> 10) / RNA / RNA (> 10) / RNA (> 100)![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Klein, D.J. / Ferre-D'Amare, A.R. | ||||||
![]() | ![]() Title: Structural basis of glmS ribozyme activation by glucosamine-6-phosphate Authors: Klein, D.J. / Ferre-D'Amare, A.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.1 KB | Display | ![]() |
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PDB format | ![]() | 67.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 416.3 KB | Display | ![]() |
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Full document | ![]() | 421.2 KB | Display | |
Data in XML | ![]() | 7.9 KB | Display | |
Data in CIF | ![]() | 10.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2gcsC ![]() 2gcvC ![]() 2h0wC ![]() 2h0xC ![]() 2h0zC ![]() 2ho6C ![]() 2ho7C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a monomer |
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Components
#1: DNA/RNA hybrid | Mass: 9048.271 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence occurs naturally in Thermoanaerobacter tengcongensis | ||
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#2: RNA chain | Mass: 40598.070 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA produced by in vitro transcription. This sequence occurs naturally in Thermoanaerobacter tengcongensis References: GenBank: 20517198 | ||
#3: Chemical | ChemComp-MG / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.68 Å3/Da / Density % sol: 54.06 % | ||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 14% PEG 4000, 1M LiCl, 100mM MES-NaOH pH 5.6, 5mM MgCl2, 4mM GlcN6P, VAPOR DIFFUSION, SITTING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 1, 2006 |
Radiation | Monochromator: Double-crystal, Si(111) liquid N2 cooled / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2155 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→30 Å / Num. all: 21613 / Num. obs: 21613 / % possible obs: 93.3 % |
Reflection shell | Resolution: 2.35→2.43 Å / % possible all: 63 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: A solvent density level of 0.35 and solvent B-factor of 20 were used for the bulk solvent during refinement | |||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.35→30 Å
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Refine LS restraints |
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