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Yorodumi- PDB-2gu9: Crystal structure of XC5357 from Xanthomonas campestris: A putati... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2gu9 | ||||||
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Title | Crystal structure of XC5357 from Xanthomonas campestris: A putative tetracenomycin polyketide synthesis protein adopting a novel cupin subfamily structure | ||||||
Components | tetracenomycin polyketide synthesis protein | ||||||
Keywords | IMMUNE SYSTEM / Xanthomonas campestris / cupin / tetracenomycin polyketide | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Xanthomonas campestris (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 1.4 Å | ||||||
Authors | Chin, K.-H. / Chou, C.C. / Wang, A.H.-J. / Chou, S.-H. | ||||||
Citation | Journal: Proteins / Year: 2006 Title: Crystal structure of XC5357 from Xanthomonas campestris: A putative tetracenomycin polyketide synthesis protein adopting a novel cupin subfamily structure Authors: Chin, K.-H. / Chou, C.C. / Wang, A.H.-J. / Chou, S.-H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2gu9.cif.gz | 50.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2gu9.ent.gz | 37.4 KB | Display | PDB format |
PDBx/mmJSON format | 2gu9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2gu9_validation.pdf.gz | 370.7 KB | Display | wwPDB validaton report |
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Full document | 2gu9_full_validation.pdf.gz | 372.5 KB | Display | |
Data in XML | 2gu9_validation.xml.gz | 5.6 KB | Display | |
Data in CIF | 2gu9_validation.cif.gz | 8.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gu/2gu9 ftp://data.pdbj.org/pub/pdb/validation_reports/gu/2gu9 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12178.454 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Xanthomonas campestris (bacteria) / Plasmid: pET30 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q8PBM3 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 12 |
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-Sample preparation
Crystal | Density Matthews: 2.96 Å3/Da / Density % sol: 58.5 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1M NaOAC, 1.6M LiSO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 1.4→30 Å / Num. all: 33553 / Num. obs: 32312 / % possible obs: 96.3 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 | ||||||||||||||||||
Reflection shell | Resolution: 1.4→1.46 Å / % possible all: 96.3 |
-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 1.4→30 Å / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.4→30 Å
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Refine LS restraints | Type: c_angle_deg / Dev ideal: 0.02 |