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- PDB-2g35: NMR structure of talin-PTB in complex with PIPKI -

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Basic information

Entry
Database: PDB / ID: 2g35
TitleNMR structure of talin-PTB in complex with PIPKI
Components
  • Talin-1
  • peptide
KeywordsSTRUCTURAL PROTEIN / TALIN / PTB DOMAIN / PIPKI
Function / homology
Function and homology information


1-phosphatidylinositol-4-phosphate 5-kinase / 1-phosphatidylinositol-4-phosphate 5-kinase activity / GRB2:SOS provides linkage to MAPK signaling for Integrins / Integrin signaling / Smooth Muscle Contraction / p130Cas linkage to MAPK signaling for integrins / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / MAP2K and MAPK activation / uropod / LIM domain binding ...1-phosphatidylinositol-4-phosphate 5-kinase / 1-phosphatidylinositol-4-phosphate 5-kinase activity / GRB2:SOS provides linkage to MAPK signaling for Integrins / Integrin signaling / Smooth Muscle Contraction / p130Cas linkage to MAPK signaling for integrins / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / MAP2K and MAPK activation / uropod / LIM domain binding / Platelet degranulation / phosphatidylinositol kinase activity / vinculin binding / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / integrin activation / clathrin-dependent endocytosis / adherens junction assembly / membrane organization / phosphatidylinositol biosynthetic process / cell-substrate junction assembly / cortical actin cytoskeleton organization / phosphatidylserine binding / regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / synaptic vesicle exocytosis / Synthesis of PIPs at the plasma membrane / phosphatidylinositol phosphate biosynthetic process / phagocytic cup / synaptic vesicle endocytosis / phagocytosis / ruffle / phosphatidylinositol binding / neutrophil chemotaxis / integrin-mediated signaling pathway / adherens junction / structural constituent of cytoskeleton / platelet aggregation / ruffle membrane / cell-cell adhesion / actin filament binding / integrin binding / presynapse / Clathrin-mediated endocytosis / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / actin cytoskeleton organization / cytoskeleton / endosome membrane / phosphorylation / focal adhesion / cell surface / nucleoplasm / ATP binding / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Phosphatidylinositol-4-phosphate 5-kinase / : / Phosphatidylinositol-4-phosphate 5-kinase, core / Phosphatidylinositol-4-phosphate 5-kinase, N-terminal / Phosphatidylinositol-4-phosphate 5-Kinase / Phosphatidylinositol phosphate kinase (PIPK) domain profile. / Phosphatidylinositol phosphate kinases / : / Talin, R4 domain / Vinculin-binding site-containing domain ...Phosphatidylinositol-4-phosphate 5-kinase / : / Phosphatidylinositol-4-phosphate 5-kinase, core / Phosphatidylinositol-4-phosphate 5-kinase, N-terminal / Phosphatidylinositol-4-phosphate 5-Kinase / Phosphatidylinositol phosphate kinase (PIPK) domain profile. / Phosphatidylinositol phosphate kinases / : / Talin, R4 domain / Vinculin-binding site-containing domain / Talin, central / Talin, N-terminal F0 domain / Talin, central domain superfamily / Talin-1/2, rod-segment / Vinculin Binding Site / Talin, middle domain / N-terminal or F0 domain of Talin-head FERM / I/LWEQ domain / I/LWEQ domain superfamily / I/LWEQ domain / I/LWEQ domain profile. / I/LWEQ domain / Phosphotyrosine-binding domain / Alpha-catenin/vinculin-like superfamily / FERM domain signature 1. / FERM conserved site / FERM domain signature 2. / FERM central domain / FERM/acyl-CoA-binding protein superfamily / Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB) / PH-domain like / FERM central domain / FERM superfamily, second domain / FERM domain / FERM domain profile. / Band 4.1 domain / Band 4.1 homologues / PH-like domain superfamily / Ubiquitin-like domain superfamily / Roll / Mainly Beta
Similarity search - Domain/homology
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma / Talin-1
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodSOLUTION NMR / Distance geometry simulated annealing
AuthorsKong, X. / Wang, X. / Misra, S. / Qin, J.
CitationJournal: J.Mol.Biol. / Year: 2006
Title: Structural Basis for the Phosphorylation-regulated Focal Adhesion Targeting of Type Igamma Phosphatidylinositol Phosphate Kinase (PIPKIgamma) by Talin.
Authors: Kong, X. / Wang, X. / Misra, S. / Qin, J.
History
DepositionFeb 17, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 2, 2006Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 9, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly ...database_2 / pdbx_struct_assembly / pdbx_struct_oper_list / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Talin-1
B: peptide


Theoretical massNumber of molelcules
Total (without water)12,6262
Polymers12,6262
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 50
RepresentativeModel #11

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Components

#1: Protein Talin-1


Mass: 11556.513 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Tln1, Tln / Plasmid: pET15b / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P26039
#2: Protein/peptide peptide


Mass: 1069.084 Da / Num. of mol.: 1 / Source method: obtained synthetically / References: UniProt: O60331*PLUS

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111HN(CA)CB
121CBCA(CO)NH
131(H)CCH-TOCSY
141HNCO
151edited NOESY
161filtered NOESY

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Sample preparation

DetailsContents: 20mM phosphate buffer, 5mM Ca2+, PH 6.3, 10% D2O, 90% H2O
Solvent system: 10% D2O, 90% H2O
Sample conditionspH: 6.3 / Temperature: 298 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian INOVAVarianINOVA5001
Varian INOVAVarianINOVA6002

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Processing

NMR software
NameClassification
TALOSdata analysis
X-PLORrefinement
RefinementMethod: Distance geometry simulated annealing / Software ordinal: 1
NMR ensembleConformers calculated total number: 50 / Conformers submitted total number: 20

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