+Open data
-Basic information
Entry | Database: PDB / ID: 2fx5 | ||||||
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Title | Pseudomonas mendocina lipase | ||||||
Components | lipase | ||||||
Keywords | HYDROLASE / alpha-beta hydrolase | ||||||
Function / homology | Function and homology information Cutinase / Cutinase / Alpha/Beta hydrolase fold, catalytic domain / Alpha/Beta hydrolase fold / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | Pseudomonas mendocina (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 1.8 Å | ||||||
Authors | Bott, R. / Wu, S. | ||||||
History |
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Remark 999 | SEQUENCE THERE WAS NO SEQUENCE DATABASE REFERENCE AT TIME OF PROCESSING. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2fx5.cif.gz | 59.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2fx5.ent.gz | 46.4 KB | Display | PDB format |
PDBx/mmJSON format | 2fx5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2fx5_validation.pdf.gz | 385 KB | Display | wwPDB validaton report |
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Full document | 2fx5_full_validation.pdf.gz | 397.8 KB | Display | |
Data in XML | 2fx5_validation.xml.gz | 8.5 KB | Display | |
Data in CIF | 2fx5_validation.cif.gz | 12.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fx/2fx5 ftp://data.pdbj.org/pub/pdb/validation_reports/fx/2fx5 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 27628.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas mendocina (bacteria) / Production host: Bacillus subtilis (bacteria) References: UniProt: Q9HXQ8, UniProt: A4Y035*PLUS, triacylglycerol lipase |
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#2: Chemical | ChemComp-TLA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.17 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 50 mM sodium acetate, 18% saturated potassium tartrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å |
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Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Jan 1, 1991 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→10 Å / Num. obs: 19100 / % possible obs: 95 % / Observed criterion σ(F): 2 / Rmerge(I) obs: 0.08 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.8→10 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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