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Open data
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Basic information
Entry | Database: PDB / ID: 2ftk | ||||||
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Title | berylloflouride Spo0F complex with Spo0B | ||||||
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![]() | TRANSFERASE / Sporulation / Spo0F / Spo0B phosphorelay | ||||||
Function / homology | ![]() Transferases; Transferring phosphorus-containing groups / sporulation resulting in formation of a cellular spore / phosphorelay signal transduction system / phosphorelay sensor kinase activity / kinase activity / phosphorylation / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Varughese, K.I. | ||||||
![]() | ![]() Title: The Crystal Structure of Beryllofluoride Spo0F in Complex with the Phosphotransferase Spo0B Represents a Phosphotransfer Pretransition State. Authors: Varughese, K.I. / Tsigelny, I. / Zhao, H. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 252.6 KB | Display | ![]() |
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PDB format | ![]() | 202.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 505.8 KB | Display | ![]() |
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Full document | ![]() | 565.5 KB | Display | |
Data in XML | ![]() | 50 KB | Display | |
Data in CIF | ![]() | 66.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1f51S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22573.559 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P06535, Transferases; Transferring phosphorus-containing groups #2: Protein | Mass: 14233.564 Da / Num. of mol.: 4 / Mutation: Y13S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P06628, Transferases; Transferring phosphorus-containing groups #3: Chemical | ChemComp-MG / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion / pH: 8.1 Details: 0.5 M KCl, 25% PEG2000, 100mM Tris HCl ph8.1, VAPOR DIFFUSION, temperature 297K |
-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 14, 2005 |
Radiation | Monochromator: Flat mirror and bent monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.05→19.86 Å / Num. all: 28073 / Num. obs: 28073 / % possible obs: 98.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 3.05→3.24 Å / % possible all: 90.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1F51 Resolution: 3.05→19.86 Å / Cross valid method: R free / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.05→19.86 Å
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Refine LS restraints |
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