+Open data
-Basic information
Entry | Database: PDB / ID: 2ftk | ||||||
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Title | berylloflouride Spo0F complex with Spo0B | ||||||
Components |
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Keywords | TRANSFERASE / Sporulation / Spo0F / Spo0B phosphorelay | ||||||
Function / homology | Function and homology information Transferases; Transferring phosphorus-containing groups / sporulation resulting in formation of a cellular spore / phosphorelay sensor kinase activity / phosphorelay signal transduction system / kinase activity / phosphorylation / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.05 Å | ||||||
Authors | Varughese, K.I. | ||||||
Citation | Journal: J.Bacteriol. / Year: 2006 Title: The Crystal Structure of Beryllofluoride Spo0F in Complex with the Phosphotransferase Spo0B Represents a Phosphotransfer Pretransition State. Authors: Varughese, K.I. / Tsigelny, I. / Zhao, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ftk.cif.gz | 252.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ftk.ent.gz | 202.5 KB | Display | PDB format |
PDBx/mmJSON format | 2ftk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ft/2ftk ftp://data.pdbj.org/pub/pdb/validation_reports/ft/2ftk | HTTPS FTP |
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-Related structure data
Related structure data | 1f51S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 22573.559 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: spo0B, spo0D / Production host: Escherichia coli (E. coli) References: UniProt: P06535, Transferases; Transferring phosphorus-containing groups #2: Protein | Mass: 14233.564 Da / Num. of mol.: 4 / Mutation: Y13S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: spo0F / Production host: Escherichia coli (E. coli) References: UniProt: P06628, Transferases; Transferring phosphorus-containing groups #3: Chemical | ChemComp-MG / |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion / pH: 8.1 Details: 0.5 M KCl, 25% PEG2000, 100mM Tris HCl ph8.1, VAPOR DIFFUSION, temperature 297K |
-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-1 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 14, 2005 |
Radiation | Monochromator: Flat mirror and bent monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.05→19.86 Å / Num. all: 28073 / Num. obs: 28073 / % possible obs: 98.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 3.05→3.24 Å / % possible all: 90.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1F51 Resolution: 3.05→19.86 Å / Cross valid method: R free / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.05→19.86 Å
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Refine LS restraints |
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