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- PDB-2eko: Solution structure of RUH-073, a Pseudo Chromo Domain from Human cDNA -
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Open data
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Basic information
Entry | Database: PDB / ID: 2eko | ||||||
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Title | Solution structure of RUH-073, a Pseudo Chromo Domain from Human cDNA | ||||||
![]() | Histone acetyltransferase HTATIP | ||||||
![]() | TRANSFERASE / Chromo domain / Histone tail / Chromatin organization modifier / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() histone H2AK5 acetyltransferase activity / peptide crotonyltransferase activity / histone H2A acetyltransferase activity / positive regulation of mitotic sister chromatid segregation / positive regulation of protein acetylation / piccolo histone acetyltransferase complex / Sensing of DNA Double Strand Breaks / MSL complex / peptide butyryltransferase activity / histone H4K16 acetyltransferase activity ...histone H2AK5 acetyltransferase activity / peptide crotonyltransferase activity / histone H2A acetyltransferase activity / positive regulation of mitotic sister chromatid segregation / positive regulation of protein acetylation / piccolo histone acetyltransferase complex / Sensing of DNA Double Strand Breaks / MSL complex / peptide butyryltransferase activity / histone H4K16 acetyltransferase activity / lipid droplet disassembly / sperm DNA condensation / peptide 2-hydroxyisobutyryltransferase activity / positive regulation of attachment of mitotic spindle microtubules to kinetochore / peptidyl-lysine acetylation / positive regulation of triglyceride biosynthetic process / Swr1 complex / histone H4 acetyltransferase activity / regulation of double-strand break repair / Impaired BRCA2 binding to PALB2 / positive regulation of circadian rhythm / positive regulation of regulatory T cell differentiation / positive regulation of innate immune response / positive regulation of aggrephagy / Cardiogenesis / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / neural tube development / DNA repair-dependent chromatin remodeling / regulation of hematopoietic stem cell differentiation / Resolution of D-loop Structures through Holliday Junction Intermediates / negative regulation of interleukin-2 production / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / HDR through Single Strand Annealing (SSA) / peptide-lysine-N-acetyltransferase activity / Impaired BRCA2 binding to RAD51 / response to ionizing radiation / NuA4 histone acetyltransferase complex / mitotic spindle pole / establishment of mitotic spindle orientation / Presynaptic phase of homologous DNA pairing and strand exchange / acetyltransferase activity / positive regulation of double-strand break repair via homologous recombination / spermatid development / positive regulation of myoblast differentiation / negative regulation of double-strand break repair via homologous recombination / cellular response to glucose starvation / positive regulation of autophagy / histone acetyltransferase activity / histone acetyltransferase / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / neurogenesis / cellular response to estradiol stimulus / nucleotide-excision repair / cellular response to glucose stimulus / Nonhomologous End-Joining (NHEJ) / transcription coregulator activity / Formation of the beta-catenin:TCF transactivating complex / double-strand break repair via homologous recombination / G2/M DNA damage checkpoint / HDR through Homologous Recombination (HRR) / DNA Damage/Telomere Stress Induced Senescence / kinetochore / cellular senescence / nucleosome / double-strand break repair / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / site of double-strand break / HATs acetylate histones / Processing of DNA double-strand break ends / regulation of apoptotic process / proteasome-mediated ubiquitin-dependent protein catabolic process / DNA-binding transcription factor binding / Estrogen-dependent gene expression / Regulation of TP53 Activity through Phosphorylation / transcription regulator complex / transcription coactivator activity / regulation of cell cycle / intracellular membrane-bounded organelle / innate immune response / negative regulation of DNA-templated transcription / apoptotic process / DNA damage response / chromatin binding / chromatin / nucleolus / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Ruhul Momen, A.Z.M. / Hirota, H. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: Solution structure of RUH-073, a Pseudo Chromo Domain from Human cDNA Authors: Ruhul Momen, A.Z.M. / Hirota, H. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 494 KB | Display | ![]() |
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PDB format | ![]() | 411.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 344.5 KB | Display | ![]() |
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Full document | ![]() | 495.3 KB | Display | |
Data in XML | ![]() | 35.1 KB | Display | |
Data in CIF | ![]() | 53.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 9915.141 Da / Num. of mol.: 1 Fragment: Histone tail binding domain, Pseudo Chromo Domain, UNP residues 5-78 Mutation: K55R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q92993, histone acetyltransferase, Transferases; Acyltransferases; Transferring groups other than aminoacyl groups |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1.19mM Domain U-15N, 13C; 20mM d-Tris-HCl (pH7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O, 90% H2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM NaCl / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |