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Yorodumi- PDB-2dvu: Crystal Structure of 2,6-Dihydroxybenzoate Decarboxylase Complexe... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2dvu | ||||||
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Title | Crystal Structure of 2,6-Dihydroxybenzoate Decarboxylase Complexed with 2,6-Dihydroxybenzoate | ||||||
Components | Thermophilic reversible gamma-resorcylate decarboxylase | ||||||
Keywords | LYASE / TIM BARREL | ||||||
Function / homology | Function and homology information gamma-resorcylate decarboxylase / organic substance metabolic process / carboxy-lyase activity / hydrolase activity / metal ion binding Similarity search - Function | ||||||
Biological species | Rhizobium sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Goto, M. | ||||||
Citation | Journal: To be Published Title: Crystal Structures of Nonoxidative Zn-Dependent 2,6-Dihydroxybenzoate (gamma-Resorcylate) Decarboxylase from Rhizobium sp. Strain MTP-10005 Authors: Goto, M. / Hayashi, H. / Miyahara, I. / Hirotsu, K. / Yoshida, M. / Oikawa, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2dvu.cif.gz | 285.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2dvu.ent.gz | 229.8 KB | Display | PDB format |
PDBx/mmJSON format | 2dvu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dv/2dvu ftp://data.pdbj.org/pub/pdb/validation_reports/dv/2dvu | HTTPS FTP |
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-Related structure data
Related structure data | 2dvtSC 2dvxC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a tetramer same as the asymmetric unit of a crystal structure. |
-Components
#1: Protein | Mass: 37468.430 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Rhizobium sp. (bacteria) / Strain: MTP-10005 References: UniProt: Q60GU1, Lyases; Carbon-carbon lyases; Carboxy-lyases #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-GRE / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.46 Å3/Da / Density % sol: 50.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 8000, HEPES-Na, Ethyleneglycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jun 8, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40.226 Å / Num. all: 116818 / Num. obs: 116804 / % possible obs: 100 % / Biso Wilson estimate: 14.2 Å2 |
Reflection shell | Resolution: 1.9→1.97 Å / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2DVT Resolution: 1.9→40.22 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 3168539.87 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 39.911 Å2 / ksol: 0.326022 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→40.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.02 Å / Rfactor Rfree error: 0.005 / Total num. of bins used: 6
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Xplor file |
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