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Yorodumi- PDB-2dlx: Solution structure of the UAS domain of human UBX domain-containi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2dlx | ||||||
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Title | Solution structure of the UAS domain of human UBX domain-containing protein 7 | ||||||
Components | UBX domain-containing protein 7 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / UAS domain / UBX domain-containing protein 7 / Protein KIAA0794 / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information VCP-NPL4-UFD1 AAA ATPase complex / ubiquitin binding / KEAP1-NFE2L2 pathway / Neddylation / proteasome-mediated ubiquitin-dependent protein catabolic process / RNA polymerase II-specific DNA-binding transcription factor binding / ubiquitin protein ligase binding / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Zhang, H.P. / Nagasima, T. / Hayashi, F. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: to be published Title: Solution structure of the UAS domain of human UBX domain-containing protein 7 Authors: Zhang, H.P. / Nagashima, T. / Hayashi, F. / Yokoyama, S. | ||||||
History |
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Remark 650 | HELIX Determination method: Author determined |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2dlx.cif.gz | 916.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2dlx.ent.gz | 799.7 KB | Display | PDB format |
PDBx/mmJSON format | 2dlx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/dl/2dlx ftp://data.pdbj.org/pub/pdb/validation_reports/dl/2dlx | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 17337.371 Da / Num. of mol.: 1 / Fragment: UAS domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: cell free protein synthesis / Gene: UBXD7, KIAA0794 / Plasmid: P050719-12 / Production host: Cell free synthesis / References: UniProt: O94888 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: spectrometer_id 1 for 3D_15N_separated_NOESY; spectrometer_id 2 for 3D_13C_separated_NOESY |
-Sample preparation
Details | Contents: 0.45 mM 13C, 15N-labeled protein; 20mM d-Tris-HCl(pH7.0); 100mM NaCl; 1mM d-DTT; 0.02%NaN3, 90%H2O; 10%D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the lowest energy, structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |