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- PDB-2dj9: Solution structure of growth-blocking peptide of the cabbage army... -

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Basic information

Entry
Database: PDB / ID: 2dj9
TitleSolution structure of growth-blocking peptide of the cabbage armyworm, Mamestra brassicae
Componentsgrowth-blocking peptide
KeywordsCYTOKINE / GROWTH-BLOCKING PEPTIDE
Function / homologyParalytic/GBP/PSP peptide / Paralytic/GBP/PSP peptide / : / Growth-blocking peptide
Function and homology information
Biological speciesMamestra brassicae (cabbage moth)
MethodSOLUTION NMR
AuthorsUmetsu, Y. / Kamiya, M. / Aizawa, T. / Kumaki, Y. / Demura, M. / Kawano, K.
CitationJournal: to be published
Title: Solution Structure of Growth-blocking Peptide of the Cabbage Armyworm, Mamestra brassicae
Authors: Umetsu, Y. / Kamiya, M. / Aizawa, T. / Kumaki, Y. / Demura, M. / Kawano, K.
History
DepositionMar 31, 2006Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Mar 31, 2007Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 9, 2022Group: Database references / Derived calculations
Category: database_2 / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4Nov 20, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: growth-blocking peptide


Theoretical massNumber of molelcules
Total (without water)2,5091
Polymers2,5091
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein/peptide growth-blocking peptide


Mass: 2508.873 Da / Num. of mol.: 1 / Fragment: residues 1-23
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mamestra brassicae (cabbage moth) / Plasmid: pET32b / Production host: Escherichia coli (E. coli) / References: GenBank: 47563661, UniProt: O96060*PLUS
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
111DQF-COSY
1212D TOCSY
1312D NOESY

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Sample preparation

DetailsContents: 5mM MbGBP, 90% H2O, 10% D2O, pH4.4 / Solvent system: 90% H2O, 10% D2O, pH4.4
Sample conditionspH: 4.4 / Pressure: ambient / Temperature: 283 K

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NMR measurement

NMR spectrometerType: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 500 MHz

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Processing

NMR software
NameVersionDeveloperClassification
CNS1.1Brungerstructure solution
CNS1.1Brungerrefinement
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 100 / Conformers submitted total number: 20

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