+Open data
-Basic information
Entry | Database: PDB / ID: 2d59 | ||||||
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Title | hypothetical protein from Pyrococcus horikoshii OT3 | ||||||
Components | hypothetical protein PH1109 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / coa binding / hypothetical protein / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Pyrococcus horikoshii (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å | ||||||
Authors | Hiyama, T.B. / Sekine, S. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: J.STRUCT.FUNCT.GENOM. / Year: 2006 Title: Structural basis of CoA recognition by the Pyrococcus single-domain CoA-binding proteins. Authors: Hiyama, T.B. / Zhao, M. / Kitago, Y. / Yao, M. / Sekine, S. / Terada, T. / Kuroishi, C. / Liu, Z.J. / Rose, J.P. / Kuramitsu, S. / Shirouzu, M. / Watanabe, N. / Yokoyama, S. / Tanaka, I. / Wang, B.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2d59.cif.gz | 42.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2d59.ent.gz | 30.3 KB | Display | PDB format |
PDBx/mmJSON format | 2d59.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2d59_validation.pdf.gz | 424.7 KB | Display | wwPDB validaton report |
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Full document | 2d59_full_validation.pdf.gz | 428.2 KB | Display | |
Data in XML | 2d59_validation.xml.gz | 9.3 KB | Display | |
Data in CIF | 2d59_validation.cif.gz | 12.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d5/2d59 ftp://data.pdbj.org/pub/pdb/validation_reports/d5/2d59 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 16747.432 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pyrococcus horikoshii (archaea) / Strain: OT3 / Gene: ph1109 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O58836 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.02 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 30% PEG 4000, 0.1M Tris-HCl, 1M di-ammonium hydrogen citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 25, 2005 |
Radiation | Monochromator: silicon / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→50 Å / Num. obs: 16631 / % possible obs: 99.2 % / Observed criterion σ(I): -3 / Redundancy: 4.9 % / Biso Wilson estimate: 24.8 Å2 / Rmerge(I) obs: 0.057 / Rsym value: 0.057 / Net I/σ(I): 40.861 |
Reflection shell | Resolution: 1.65→1.71 Å / Redundancy: 5.9 % / Rmerge(I) obs: 0.614 / Mean I/σ(I) obs: 3.6 / Num. unique all: 1564 / Rsym value: 0.614 / % possible all: 96.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.65→40.33 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 915614.16 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.4352 Å2 / ksol: 0.396119 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.65→40.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.65→1.75 Å / Rfactor Rfree error: 0.027 / Total num. of bins used: 6
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Xplor file |
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