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Yorodumi- PDB-2cow: Solution structure of the CAP-Gly domain in human Kinesin-like pr... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2cow | ||||||
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Title | Solution structure of the CAP-Gly domain in human Kinesin-like protein KIF13B | ||||||
Components | Kinesin-like protein KIF13B | ||||||
Keywords | PROTEIN BINDING / microtubule binding / cytoskeleton associated protein / KIAA0639 / Kinesin-like protein GAKIN / structural genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information cytoskeleton-dependent intracellular transport / Kinesins / COPI-dependent Golgi-to-ER retrograde traffic / microtubule motor activity / kinesin complex / regulation of axonogenesis / microtubule-based movement / protein targeting / 14-3-3 protein binding / T cell activation ...cytoskeleton-dependent intracellular transport / Kinesins / COPI-dependent Golgi-to-ER retrograde traffic / microtubule motor activity / kinesin complex / regulation of axonogenesis / microtubule-based movement / protein targeting / 14-3-3 protein binding / T cell activation / microtubule binding / microtubule / axon / protein kinase binding / signal transduction / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Saito, K. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the CAP-Gly domain in human Kinesin-like protein KIF13B Authors: Saito, K. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2cow.cif.gz | 562.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2cow.ent.gz | 467.4 KB | Display | PDB format |
PDBx/mmJSON format | 2cow.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/co/2cow ftp://data.pdbj.org/pub/pdb/validation_reports/co/2cow | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10483.403 Da / Num. of mol.: 1 / Fragment: CAP-Gly domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: Kazusa cDNA hj03358 / Plasmid: P040315-02 / References: UniProt: Q9NQT8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM protein, 20mM d-Tris-HCl, pH7.0, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120 / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |