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- PDB-2cbb: STRUCTURE OF NATIVE AND APO CARBONIC ANHYDRASE II AND SOME OF ITS... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2cbb | ||||||
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Title | STRUCTURE OF NATIVE AND APO CARBONIC ANHYDRASE II AND SOME OF ITS ANION-LIGAND COMPLEXES | ||||||
![]() | CARBONIC ANHYDRASE II | ||||||
![]() | LYASE(OXO-ACID) | ||||||
Function / homology | ![]() positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway ...positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway / positive regulation of synaptic transmission, GABAergic / morphogenesis of an epithelium / regulation of intracellular pH / carbonic anhydrase / carbonate dehydratase activity / carbon dioxide transport / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / neuron cellular homeostasis / one-carbon metabolic process / apical part of cell / myelin sheath / extracellular exosome / zinc ion binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Hakansson, K. / Carlsson, M. / Svensson, L.A. / Liljas, A. | ||||||
![]() | ![]() Title: Structure of native and apo carbonic anhydrase II and structure of some of its anion-ligand complexes. Authors: Hakansson, K. / Carlsson, M. / Svensson, L.A. / Liljas, A. #1: ![]() Title: Structure of Cobalt Carbonic Anhydrase Complexed with Bicarbonate Authors: Hakansson, K. / Wehnert, A. | ||||||
History |
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Remark 700 | SHEET SHEET B1 OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET ...SHEET SHEET B1 OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS *B1A* AND *B1B* ARE DEFINED. STRANDS 5, 6, 7, 8, 9, AND 10 OF B1A ARE IDENTICAL TO STRANDS 2, 3, 4, 5, 6, AND 7 OF B1B, RESPECTIVELY. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.7 KB | Display | ![]() |
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PDB format | ![]() | 50.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES 4, 14, 64, AND 136 ARE DISORDERED AND ARE PRESENTED WITH TWO ALTERNATE CONFORMATIONS IN THIS ENTRY. 2: RESIDUES 30 AND 202 ARE CIS PROLINES. |
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Components
#1: Protein | Mass: 29183.902 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() | ||
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#2: Chemical | ChemComp-ZN / | ||
#3: Water | ChemComp-HOH / | ||
Nonpolymer details | CARBONIC ANHYDRASE CONTAINS ONE ZINC ION PER POLYPEPTIDSequence details | RESIDUES 125 AND 127 ARE ADJACENT IN THE SEQUENCE. | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.89 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 8.5 / Method: microdialysis | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.67 Å / Lowest resolution: 3.03 Å / Num. all: 29394 / Num. obs: 25425 / % possible obs: 86.5 % / Num. measured all: 71288 / Rmerge(I) obs: 0.054 |
Reflection shell | *PLUS Highest resolution: 1.67 Å / Lowest resolution: 1.77 Å / % possible obs: 86.5 % / Num. possible: 4843 / Num. unique obs: 2337 / Num. measured obs: 3925 / Rmerge(I) obs: 0.144 / Mean I/σ(I) obs: 38.3 |
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Processing
Software | Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.67→10 Å /
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Refinement step | Cycle: LAST / Resolution: 1.67→10 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.67 Å / Lowest resolution: 10 Å / Num. reflection obs: 25425 / Rfactor obs: 0.153 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |