SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AB" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 203 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, CYS 15 TO ALA ENGINEERED RESIDUE IN CHAIN A, GLN 16 TO ALA ...ENGINEERED RESIDUE IN CHAIN A, CYS 15 TO ALA ENGINEERED RESIDUE IN CHAIN A, GLN 16 TO ALA ENGINEERED RESIDUE IN CHAIN A, ALA 17 TO VAL
Sequence details
THE CONFLICTS SHOWN IN THE SEQADV RECORDS BELOW ARE NOT INTENTIONAL MUTATIONS, AND THE AUTHORS ...THE CONFLICTS SHOWN IN THE SEQADV RECORDS BELOW ARE NOT INTENTIONAL MUTATIONS, AND THE AUTHORS CANNOT SAY WHETHER THIS IS AN ERROR IN THE CONSTRUCT OR IN THE ORIGINAL SEQUENCE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.3 Å3/Da / Density % sol: 44.9 %
Crystal grow
pH: 6.5 Details: 0.1 M MES PH6.5, 0.2 M AMMONIUM SULPHATE, 25%(W/V) MME PEG 5000, 3% DIOXANE, pH 6.50