+Open data
-Basic information
Entry | Database: PDB / ID: 2bkn | ||||||
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Title | STRUCTURE ANALYSIS OF UNKNOWN FUNCTION PROTEIN | ||||||
Components | HYPOTHETICAL PROTEIN PH0236Hypothesis | ||||||
Keywords | MEMBRANE PROTEIN / HELIX RICH | ||||||
Function / homology | Function and homology information monoatomic cation transmembrane transporter activity / potassium ion transport / metal ion binding Similarity search - Function | ||||||
Biological species | PYROCOCCUS HORIKOSHII (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.6 Å | ||||||
Authors | Inagaki, E. / Tahirov, T.H. | ||||||
Citation | Journal: To be Published Title: Crystal Analysis of Putative Potassium Channel Related Protein from Pyrococcus Horikoshii Authors: Inagaki, E. / Tahirov, T.H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bkn.cif.gz | 51.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bkn.ent.gz | 40 KB | Display | PDB format |
PDBx/mmJSON format | 2bkn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bk/2bkn ftp://data.pdbj.org/pub/pdb/validation_reports/bk/2bkn | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 23012.564 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PYROCOCCUS HORIKOSHII (archaea) / Strain: OT3 / Plasmid: PET11A / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O57975 | ||
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#2: Chemical | ChemComp-CS / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.43 % |
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Crystal grow | pH: 4.7 Details: ROTEIN WAS CRYSTALLIZED FROM 1.25 M SODIUM CHLORIDE, 50 MM ACATATE, PH 4.7; THEN SOAKED IN 1.5 M CSCL. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B1 / Wavelength: 1 |
Detector | Type: RIGAKU IMAGE PLATE / Detector: IMAGE PLATE / Date: Oct 30, 2003 / Details: MIRRORS |
Radiation | Monochromator: SILICON CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→35 Å / Num. obs: 8141 / % possible obs: 98.8 % / Observed criterion σ(I): -1 / Redundancy: 4.6 % / Biso Wilson estimate: 32.9 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 3.4 / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: OTHER / Resolution: 2.6→33.45 Å / Rfactor Rfree error: 0.012 / Data cutoff high absF: 359332.47 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: THE OCCUPANCIES OF SOME CESIUM IONS ARE NOT ONE.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.1 Å2 / ksol: 0.378279 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.6→33.45 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.6→2.76 Å / Rfactor Rfree error: 0.041 / Total num. of bins used: 6
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Xplor file |
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