+Open data
-Basic information
Entry | Database: PDB / ID: 2bjx | ||||||
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Title | PROTEIN DISULFIDE ISOMERASE | ||||||
Components | PROTEIN (PROTEIN DISULFIDE ISOMERASE) | ||||||
Keywords | ELECTRON TRANSPORT / REDOX-ACTIVE CENTER / ISOMERASE / ENDOPLASMIC RETICULUM | ||||||
Function / homology | Function and homology information regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / procollagen-proline 4-dioxygenase complex / VLDL assembly / insulin processing / procollagen-proline 4-dioxygenase activity / interleukin-23-mediated signaling pathway / LDL remodeling / thiol oxidase activity / protein disulfide-isomerase / peptidyl-proline hydroxylation to 4-hydroxy-L-proline ...regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / procollagen-proline 4-dioxygenase complex / VLDL assembly / insulin processing / procollagen-proline 4-dioxygenase activity / interleukin-23-mediated signaling pathway / LDL remodeling / thiol oxidase activity / protein disulfide-isomerase / peptidyl-proline hydroxylation to 4-hydroxy-L-proline / endoplasmic reticulum chaperone complex / Chylomicron assembly / protein folding in endoplasmic reticulum / Collagen biosynthesis and modifying enzymes / Interleukin-23 signaling / interleukin-12-mediated signaling pathway / cellular response to interleukin-7 / Interleukin-12 signaling / Insulin processing / protein disulfide isomerase activity / Detoxification of Reactive Oxygen Species / positive regulation of cell adhesion / protein-disulfide reductase activity / endoplasmic reticulum-Golgi intermediate compartment / endoplasmic reticulum to Golgi vesicle-mediated transport / positive regulation of substrate adhesion-dependent cell spreading / response to endoplasmic reticulum stress / Post-translational protein phosphorylation / Hedgehog ligand biogenesis / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / melanosome / integrin binding / protein folding / lamellipodium / actin binding / cellular response to hypoxia / positive regulation of viral entry into host cell / cytoskeleton / protein heterodimerization activity / endoplasmic reticulum lumen / external side of plasma membrane / focal adhesion / enzyme binding / endoplasmic reticulum / protein-containing complex / RNA binding / extracellular exosome / extracellular region / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, SIMULATED ANNEALING | ||||||
Authors | Kemmink, J. / Dijkstra, K. / Mariani, M. / Scheek, R.M. / Penka, E. / Nilges, M. / Darby, N.J. | ||||||
Citation | Journal: J.Biomol.NMR / Year: 1999 Title: The structure in solution of the b domain of protein disulfide isomerase. Authors: Kemmink, J. / Dijkstra, K. / Mariani, M. / Scheek, R.M. / Penka, E. / Nilges, M. / Darby, N.J. #1: Journal: Curr.Biol. / Year: 1997 Title: The Folding Catalyst Protein Disulfide Isomerase is Constructed of Active and Inactive Thioredoxin Modules Authors: Kemmink, J. / Darby, N.J. / Dijkstra, K. / Nilges, M. / Creighton, T.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2bjx.cif.gz | 774.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2bjx.ent.gz | 650 KB | Display | PDB format |
PDBx/mmJSON format | 2bjx.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bj/2bjx ftp://data.pdbj.org/pub/pdb/validation_reports/bj/2bjx | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12072.295 Da / Num. of mol.: 1 / Fragment: B DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Strain: BL21 (DE3) / Plasmid: PET12A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P07237, protein disulfide-isomerase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Sample conditions | pH: 6.5 / Pressure: 1 atm / Temperature: 300 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DISTANCE GEOMETRY, SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAIL CAN BE FOUND IN THE JRNL CITATION | |||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 24 |