温度: 300 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7.5 詳細: NSUN5 was complexed with S-adenosyl-L-methionine (SAM) (Sigma) at 1:10 molar ratio of protein :SAM and crystallized using the sitting drop vapor diffusion method at 20 C by mixing 1 l of the ...詳細: NSUN5 was complexed with S-adenosyl-L-methionine (SAM) (Sigma) at 1:10 molar ratio of protein :SAM and crystallized using the sitting drop vapor diffusion method at 20 C by mixing 1 l of the protein solution with 1 l of the reservoir solution containing 10% Iso-propanole, 20% PEG 4000, 0.1M Na HEPES pH 7.5. , VAPOR DIFFUSION, HANGING DROP, temperature 300K
解像度: 1.65→59.55 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.94 / SU B: 1.638 / SU ML: 0.058 / 交差検証法: THROUGHOUT / ESU R: 0.095 / ESU R Free: 0.097 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.22226
2038
5 %
RANDOM
Rwork
0.18624
-
-
-
obs
0.18807
38415
99.75 %
-
all
-
38415
-
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK