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Yorodumi- PDB-2b8f: solution structure of Bacillus subtilis BLAP Apo form (energy min... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2b8f | ||||||
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Title | solution structure of Bacillus subtilis BLAP Apo form (energy minimized mean structure) | ||||||
Components | Biotin/Lipoyl Attachment Protein | ||||||
Keywords | BIOSYNTHETIC PROTEIN / Bacillus subtilis / Single-domain Biotin Carboxyl Carrier Protein / solution structure | ||||||
Function / homology | Function and homology information : / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Biotin-requiring enzyme / Biotinyl/lipoyl domain profile. / Biotin/lipoyl attachment / Single hybrid motif / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta Similarity search - Domain/homology | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | SOLUTION NMR / smulated annealing molecular dynamics | ||||||
Model type details | minimized average | ||||||
Authors | Cui, G. / Xia, B. / Jin, C. | ||||||
Citation | Journal: To be published Title: solution structure of Bacillus subtilis BLAP Apo form (energy minimized mean structure) Authors: Cui, G. / Xia, B. / Jin, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2b8f.cif.gz | 32.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2b8f.ent.gz | 21.8 KB | Display | PDB format |
PDBx/mmJSON format | 2b8f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b8/2b8f ftp://data.pdbj.org/pub/pdb/validation_reports/b8/2b8f | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7920.094 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Plasmid: pET-21a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pLysS / References: UniProt: Q9R9I3, UniProt: C0H419*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2mM S-BCCP, U-15N, 13C; 50mM phosphate buffer NA; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 150 mM / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: smulated annealing molecular dynamics / Software ordinal: 1 Details: the structures are based on a total of 4125 restraints. 3731 are NOE-derived distance constraints, 109 dihedral angle restraints,28 distance restraints from hydrogen bonds, 257 charity restraints | ||||||||||||||||||||||||
NMR representative | Selection criteria: minimized average structure | ||||||||||||||||||||||||
NMR ensemble | Conformers submitted total number: 1 |