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Yorodumi- PDB-2ayy: Solution structure of the E.coli RcsC C-terminus (residues 700-81... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ayy | ||||||
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Title | Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region | ||||||
Components | Sensor kinase protein rcsC | ||||||
Keywords | TRANSFERASE / NEW DOMAIN / ALPHA/BETA LAYER | ||||||
Function / homology | Function and homology information peptidyl-aspartic acid phosphorylation / peptidyl-histidine phosphorylation / protein histidine kinase activity / histidine phosphotransfer kinase activity / single-species biofilm formation / cellular response to osmotic stress / histidine kinase / phosphorelay signal transduction system / plasma membrane => GO:0005886 / phosphoprotein phosphatase activity ...peptidyl-aspartic acid phosphorylation / peptidyl-histidine phosphorylation / protein histidine kinase activity / histidine phosphotransfer kinase activity / single-species biofilm formation / cellular response to osmotic stress / histidine kinase / phosphorelay signal transduction system / plasma membrane => GO:0005886 / phosphoprotein phosphatase activity / phosphorelay sensor kinase activity / outer membrane-bounded periplasmic space / protein autophosphorylation / regulation of DNA-templated transcription / ATP binding / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | SOLUTION NMR / Energy minimization | ||||||
Authors | Rogov, V.V. / Rogova, N.Y. / Bernhard, F. / Koglin, A. / Lohr, F. / Dotsch, V. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: A New Structural Domain in the Escherichia coli RcsC Hybrid Sensor Kinase Connects Histidine Kinase and Phosphoreceiver Domains Authors: Rogov, V.V. / Rogova, N.Y. / Bernhard, F. / Koglin, A. / Lohr, F. / Dotsch, V. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ayy.cif.gz | 888.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ayy.ent.gz | 757 KB | Display | PDB format |
PDBx/mmJSON format | 2ayy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ayy_validation.pdf.gz | 349.2 KB | Display | wwPDB validaton report |
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Full document | 2ayy_full_validation.pdf.gz | 540.6 KB | Display | |
Data in XML | 2ayy_validation.xml.gz | 45.4 KB | Display | |
Data in CIF | 2ayy_validation.cif.gz | 77.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ay/2ayy ftp://data.pdbj.org/pub/pdb/validation_reports/ay/2ayy | HTTPS FTP |
-Related structure data
Related structure data | 2ayxC 2ayzC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13214.005 Da / Num. of mol.: 1 / Fragment: U-domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: RCSC / Plasmid: pQE60 / Production host: Escherichia coli (E. coli) / Strain (production host): M15 References: UniProt: P14376, UniProt: P0DMC5*PLUS, Transferases; Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: The 3D_13C-Separated_NOESY was recorded in three modifications |
-Sample preparation
Details |
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Sample conditions | pH: 7.5 / Pressure: 1 atm / Temperature: 296 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: Energy minimization / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy and fewest violation | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 25 |