+Open data
-Basic information
Entry | Database: PDB / ID: 2aqa | ||||||
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Title | NMR structural analysis of Nop10p from Saccharomyces cerevisiae | ||||||
Components | H/ACA ribonucleoprotein complex subunit 3 | ||||||
Keywords | RNA BINDING PROTEIN / Nop10p | ||||||
Function / homology | Function and homology information snoRNA guided rRNA pseudouridine synthesis / box H/ACA snoRNP complex / rRNA pseudouridine synthesis / snRNA pseudouridine synthesis / sno(s)RNA-containing ribonucleoprotein complex / telomerase RNA binding / snoRNA binding / rRNA processing / nucleolus / RNA binding Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Hamma, T. / Reichow, S.L. / Varani, G. / Ferre-D'Amare, A.R. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2005 Title: The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs. Authors: Hamma, T. / Reichow, S.L. / Varani, G. / Ferre-D'Amare, A.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2aqa.cif.gz | 420.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2aqa.ent.gz | 370.7 KB | Display | PDB format |
PDBx/mmJSON format | 2aqa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/aq/2aqa ftp://data.pdbj.org/pub/pdb/validation_reports/aq/2aqa | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7347.430 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Gene: NOP10 / Plasmid: pET9 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta pLys S / References: UniProt: Q6Q547 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: Dihedral Restraints Derived from Talos Predicitons |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 50mM; 100mM / pH: 5.0 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: the structures are based on a total of 467 restraints, 422 are NOE-derived distance constraints, 45 dihedral angle restraints | ||||||||||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |