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- PDB-2anj: Crystal Structure of the Glur2 Ligand Binding Core (S1S2J-Y450W) ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2anj | ||||||
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Title | Crystal Structure of the Glur2 Ligand Binding Core (S1S2J-Y450W) Mutant in Complex With the Partial Agonist Kainic Acid at 2.1 A Resolution | ||||||
![]() | Glutamate receptor 2 | ||||||
![]() | MEMBRANE PROTEIN / Ionotropic Glutamate Receptor / GluR2 Ligand-Binding Core / Agonist Complex / Mutant | ||||||
Function / homology | ![]() spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / ligand-gated monoatomic cation channel activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding ...spine synapse / dendritic spine neck / dendritic spine head / Activation of AMPA receptors / perisynaptic space / AMPA glutamate receptor activity / ligand-gated monoatomic cation channel activity / Trafficking of GluR2-containing AMPA receptors / response to lithium ion / immunoglobulin binding / AMPA glutamate receptor complex / kainate selective glutamate receptor activity / ionotropic glutamate receptor complex / extracellularly glutamate-gated ion channel activity / cellular response to glycine / asymmetric synapse / regulation of receptor recycling / Unblocking of NMDA receptors, glutamate binding and activation / positive regulation of synaptic transmission / glutamate receptor binding / extracellular ligand-gated monoatomic ion channel activity / glutamate-gated receptor activity / glutamate-gated calcium ion channel activity / presynaptic active zone membrane / response to fungicide / regulation of synaptic transmission, glutamatergic / somatodendritic compartment / cellular response to brain-derived neurotrophic factor stimulus / dendrite membrane / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / cytoskeletal protein binding / ionotropic glutamate receptor binding / dendrite cytoplasm / ionotropic glutamate receptor signaling pathway / SNARE binding / dendritic shaft / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / synaptic membrane / synaptic transmission, glutamatergic / PDZ domain binding / protein tetramerization / postsynaptic density membrane / modulation of chemical synaptic transmission / establishment of protein localization / Schaffer collateral - CA1 synapse / terminal bouton / receptor internalization / cerebral cortex development / synaptic vesicle membrane / synaptic vesicle / presynapse / signaling receptor activity / presynaptic membrane / amyloid-beta binding / growth cone / scaffold protein binding / chemical synaptic transmission / postsynaptic membrane / perikaryon / dendritic spine / postsynaptic density / neuron projection / axon / neuronal cell body / glutamatergic synapse / synapse / dendrite / protein-containing complex binding / protein kinase binding / cell surface / endoplasmic reticulum / protein-containing complex / identical protein binding / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Holm, M.M. / Naur, P. / Vestergaard, B. / Geballe, M.T. / Gajhede, M. / Kastrup, J.S. / Traynelis, S.F. / Egebjerg, J. | ||||||
![]() | ![]() Title: A Binding Site Tyrosine Shapes Desensitization Kinetics and Agonist Potency at GluR2: a mutagenic, kinetic, and crystallographic study Authors: Holm, M.M. / Naur, P. / Vestergaard, B. / Geballe, M.T. / Gajhede, M. / Kastrup, J.S. / Traynelis, S.F. / Egebjerg, J. #1: ![]() Title: Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core. Authors: Armstrong, N. / Gouaux, E. #2: ![]() Title: Tyr702 is an important determinant of agonist binding and domain closure of the ligand-binding core of GluR2. Authors: Frandsen, A. / Pickering, D.S. / Vestergaard, B. / Kasper, C. / Nielsen, B.B. / Greenwood, J.R. / Campiani, G. / Fattorusso, C. / Gajhede, M. / Schousboe, A. / Kastrup, J.S. #3: ![]() Title: Tuning Activation of the Ampa-Sensitive Glur2 Ion Channel by Genetic Adjustment of Agonist-Induced Authors: Armstrong, N. / Mayer, M. / Gouaux, E. | ||||||
History |
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Remark 999 | SEQUENCE Native GluR2 is a membrane protein. The protein crystallized is the extracellular ligand ...SEQUENCE Native GluR2 is a membrane protein. The protein crystallized is the extracellular ligand binding domain of GluR2. Transmembrane regions were genetically removed and replaced with a Gly-Thr linker (residues 118-119). |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 72.1 KB | Display | ![]() |
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PDB format | ![]() | 51.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1ftkS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The intact receptor is a tetramer consisting of a dimer-of-dimers. Only the dimer can be found in the crystal by applying the following transformation to chain A: -1 0 0 0 -1 0 0 0 1 1 0 0 |
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Components
#1: Protein | Mass: 29244.721 Da / Num. of mol.: 1 / Fragment: Ligand Binding Core / Mutation: Y61W Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-KAI / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 50.15 % |
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Crystal grow | Temperature: 279 K / pH: 6.5 Details: PEG 8000, Cacodylate, Lithium Sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K, pH 6.50 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 9, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.027 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→25 Å / Num. obs: 16201 / % possible obs: 92.9 % / Observed criterion σ(I): -3 / Redundancy: 3.8 % / Biso Wilson estimate: 17.15 Å2 / Rsym value: 0.107 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.1→2.18 Å / Redundancy: 3.7 % / Mean I/σ(I) obs: 4.5 / Rsym value: 0.292 / % possible all: 94 |
-Phasing
Phasing MR | Rfactor: 51.9 / Cor.coef. Fo:Fc: 29.7 / Cor.coef. Io to Ic: 17.9
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1FTK Resolution: 2.1→25 Å / Rfactor Rfree error: 0.012 / Data cutoff high absF: 290292.49 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER Details: RESIDUE 263 COULD NOT BE MODELLED INTO THE ELECTRON DENSITY.
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.37 Å2 / ksol: 0.370341 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 15.72 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.034 / Total num. of bins used: 6
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