+
Open data
-
Basic information
Entry | Database: PDB / ID: 2aef | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structures of the MthK RCK Domain in Ca2+ bound form | ||||||
![]() | Calcium-gated potassium channel mthK | ||||||
![]() | METAL TRANSPORT / membrane protein / Rossmann fold / helix-turn-helix / Ca2+ binding / flexible interface / bilobed architecture | ||||||
Function / homology | ![]() monoatomic cation transmembrane transporter activity / potassium ion transport / identical protein binding / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Dong, J. / Shi, N. / Berke, I. / Chen, L. / Jiang, Y. | ||||||
![]() | ![]() Title: Structures of the MthK RCK Domain and the Effect of Ca2+ on Gating Ring Stability Authors: Dong, J. / Shi, N. / Berke, I. / Chen, L. / Jiang, Y. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 111.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 83.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.3 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 454.1 KB | Display | |
Data in XML | ![]() | 25.3 KB | Display | |
Data in CIF | ![]() | 37.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2aejC ![]() 2aemC ![]() 1lnqS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 25914.584 Da / Num. of mol.: 2 / Fragment: isoform Soluble Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: mthK / Plasmid: pQE70 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 40 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: PEG 4000, calcium chloride, Ammonium formate, sodium acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 1, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9793 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→29.2 Å / Num. all: 46611 / Num. obs: 46246 / % possible obs: 99.2 % / Observed criterion σ(I): 0 / Redundancy: 4.1 % / Rmerge(I) obs: 0.03 / Rsym value: 0.03 / Net I/σ(I): 40.8 |
Reflection shell | Resolution: 1.7→1.76 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.03 / Num. unique all: 4521 / Rsym value: 0.282 / % possible all: 98.1 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: pdb entry 1LNQ Resolution: 1.7→29.2 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→29.2 Å
|