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Yorodumi- PDB-2ab9: Discovery, structural determination and processing of the precurs... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ab9 | ||||||
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Title | Discovery, structural determination and processing of the precursor protein that produces the cyclic trypsin inhibitor SFTI-1 | ||||||
Components | pro-SFTI-1 | ||||||
Keywords | HYDROLASE INHIBITOR / Protein / beta-sheet / random coil | ||||||
Function / homology | negative regulation of endopeptidase activity / endopeptidase inhibitor activity / serine-type endopeptidase inhibitor activity / protease binding / Trypsin inhibitor 1 Function and homology information | ||||||
Biological species | Helianthus annuus (common sunflower) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, simulated annealing | ||||||
Authors | Mulvenna, J.P. / Foley, F.M. / Craik, D.J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: Discovery, structural determination and putative processing of the precursor protein that produces the cyclic trypsin inhibitor SFTI-1 Authors: Mulvenna, J.P. / Foley, F.M. / Craik, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ab9.cif.gz | 184.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ab9.ent.gz | 159.7 KB | Display | PDB format |
PDBx/mmJSON format | 2ab9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ab9_validation.pdf.gz | 341.8 KB | Display | wwPDB validaton report |
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Full document | 2ab9_full_validation.pdf.gz | 431.5 KB | Display | |
Data in XML | 2ab9_validation.xml.gz | 11.3 KB | Display | |
Data in CIF | 2ab9_validation.cif.gz | 18.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ab/2ab9 ftp://data.pdbj.org/pub/pdb/validation_reports/ab/2ab9 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3371.840 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Helianthus annuus (common sunflower) / Plasmid: pTWIN1 / Production host: Escherichia coli (E. coli) / Strain (production host): ER2566 / References: UniProt: Q4GWU5 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: NA / pH: 4 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |