PDB-22vh: The assembled CPC trimer

ID or keywords:

Entry

Database: PDB / ID: 22vh

Title

The assembled CPC trimer

Components

C-phycocyanin alpha subunit
C-phycocyanin beta subunit

Keywords

PHOTOSYNTHESIS / Phycocyanin / light-harvesting / energy-transfer

Function / homology

Function and homology information

phycobilisome / plasma membrane-derived thylakoid membrane / photosynthesis
Similarity search - Function

Biological species

Nostoc sp. PCC 7120 = FACHB-418 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.5 Å

Authors

Zhao, x. / Zhu, j.x. / Xue, c.y. / Zheng, x.y. / Zhao, k.h.

Funding support

China, 1items

Citation

Journal: Biochim Biophys Acta Bioenerg / Year: 2026
Title: Biosynthesis of C-phycocyanin trimers.
Authors: Zhao, X. / Zhu, J.X. / Li, G.C. / Zhao, K.H.

History

Deposition	Jan 25, 2026	Deposition site: PDBJ / Processing site: PDBC
Revision 1.0	Aug 19, 2026	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	22vh.cif.gz	945.6 KB	Display	PDBx/mmCIF format
PDB format	pdb22vh.ent.gz	661.5 KB	Display	PDB format
PDBx/mmJSON format	22vh.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/2v/22vh ftp://data.pdbj.org/pub/pdb/validation_reports/2v/22vh	HTTPS FTP

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Related structure data

Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: C-phycocyanin alpha subunit

D: C-phycocyanin beta subunit

B: C-phycocyanin alpha subunit

C: C-phycocyanin beta subunit

E: C-phycocyanin alpha subunit

F: C-phycocyanin beta subunit

G: C-phycocyanin alpha subunit

H: C-phycocyanin beta subunit

I: C-phycocyanin alpha subunit

J: C-phycocyanin beta subunit

K: C-phycocyanin alpha subunit

L: C-phycocyanin beta subunit

hetero molecules

250 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: assay for oligomerization, To determine the oligomeric state of the protein, it was first purified by Ni2+-affinity chromatography, followed by size exclusion chromatography (SuperdexTM 200 ...Evidence: assay for oligomerization, To determine the oligomeric state of the protein, it was first purified by Ni2+-affinity chromatography, followed by size exclusion chromatography (SuperdexTM 200 10/300 GL) with 20 mM KPB (50 mM NaCl, pH 7.2). Molecular mass was determined using calibration curves for marker standards (Sigma-Aldrich).

Unit cell

Length a, b, c (Å)	117.970, 109.960, 182.370
Angle α, β, γ (deg.)	90.000, 103.470, 90.000
Int Tables number	5
Space group name H-M	C121
Space group name Hall	C2y
Symmetry operation	#1: x,y,z #2: -x,y,-z #3: x+1/2,y+1/2,z #4: -x+1/2,y+1/2,-z

Components on special symmetry positions

ID	Model	Components
1	1	D-353- HOH

#1: Protein	C-phycocyanin alpha subunit Mass: 21526.016 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria) Gene: cpcA, alr0529 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P07121
#2: Protein	C-phycocyanin beta subunit Mass: 18403.758 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Nostoc sp. PCC 7120 = FACHB-418 (bacteria) Gene: cpcB, alr0528 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P07120
#3: Chemical	ChemComp-CYC / PHYCOCYANOBILIN Mass: 588.694 Da / Num. of mol.: 18 / Source method: obtained synthetically / Formula: C₃₃H₄₀N₄O₆ / Feature type: SUBJECT OF INVESTIGATION
#4: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 604 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	Y
Has protein modification	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.4 Å³/Da / Density % sol: 48.76 %
Crystal grow	Temperature: 289 K / Method: vapor diffusion, sitting drop Details: 0.1 M tribasic sodium citrate dihydrate (pH 5.5) and 18% w/v polyethylene glycol 3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL02U1 / Wavelength: 0.97918 Å
Detector	Type: DECTRIS EIGER2 S 9M / Detector: PIXEL / Date: Dec 9, 2025
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97918 Å / Relative weight: 1
Reflection	Resolution: 2.5→54.27 Å / Num. obs: 78269 / % possible obs: 99.9 % / Redundancy: 6.6 % / CC_1/2: 0.999 / R_merge(I) obs: 0.28 / Net I/σ(I): 9
Reflection shell	Resolution: 2.5→2.55 Å / Num. unique obs: 6070 / CC_1/2: 0.999 / % possible all: 99.9

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
XDS		data reduction
PHASER		phasing
Aimless		data scaling

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.5→54.27 Å / SU ML: 0.2807 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.5453
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2461	3866	4.94 %
R_work	0.1936	74369	-
obs	0.1962	78235	99.81 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 35.98 Å²

Refinement step

Cycle: LAST / Resolution: 2.5→54.27 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	15206	0	774	604	16584

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0025	16246
X-RAY DIFFRACTION	f_angle_d	0.5765	22126
X-RAY DIFFRACTION	f_chiral_restr	0.034	2453
X-RAY DIFFRACTION	f_plane_restr	0.0036	2885
X-RAY DIFFRACTION	f_dihedral_angle_d	11.4711	2484

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.5-2.53	0.2756	139	0.2394	2603	X-RAY DIFFRACTION	99.89
2.53-2.56	0.3277	123	0.2377	2656	X-RAY DIFFRACTION	99.96
2.56-2.6	0.3468	154	0.2479	2671	X-RAY DIFFRACTION	99.96
2.6-2.63	0.2923	127	0.2378	2631	X-RAY DIFFRACTION	99.93
2.63-2.67	0.3012	126	0.2523	2648	X-RAY DIFFRACTION	99.96
2.67-2.71	0.3088	139	0.2381	2654	X-RAY DIFFRACTION	100
2.71-2.75	0.2893	151	0.2243	2644	X-RAY DIFFRACTION	99.96
2.75-2.8	0.2713	152	0.2274	2647	X-RAY DIFFRACTION	99.96
2.8-2.84	0.3053	151	0.24	2609	X-RAY DIFFRACTION	99.93
2.84-2.9	0.312	130	0.2325	2690	X-RAY DIFFRACTION	99.96
2.9-2.95	0.2834	132	0.2313	2653	X-RAY DIFFRACTION	99.93
2.95-3.01	0.291	118	0.2169	2652	X-RAY DIFFRACTION	100
3.01-3.08	0.2606	151	0.2262	2646	X-RAY DIFFRACTION	100
3.08-3.15	0.2824	153	0.2259	2617	X-RAY DIFFRACTION	99.96
3.15-3.23	0.2926	130	0.2334	2713	X-RAY DIFFRACTION	100
3.23-3.32	0.2613	148	0.2085	2616	X-RAY DIFFRACTION	99.78
3.32-3.41	0.2793	139	0.2117	2634	X-RAY DIFFRACTION	99.6
3.41-3.52	0.2743	130	0.1929	2690	X-RAY DIFFRACTION	99.65
3.52-3.65	0.2308	131	0.1841	2672	X-RAY DIFFRACTION	100
3.65-3.8	0.2327	140	0.1815	2656	X-RAY DIFFRACTION	99.96
3.8-3.97	0.2539	127	0.1748	2651	X-RAY DIFFRACTION	100
3.97-4.18	0.1996	146	0.1604	2657	X-RAY DIFFRACTION	99.93
4.18-4.44	0.2033	129	0.1535	2681	X-RAY DIFFRACTION	99.79
4.44-4.78	0.1873	152	0.1534	2641	X-RAY DIFFRACTION	99.15
4.78-5.26	0.2103	134	0.1706	2645	X-RAY DIFFRACTION	99.82
5.26-6.02	0.2198	151	0.1848	2688	X-RAY DIFFRACTION	100
6.02-7.58	0.2097	143	0.1698	2688	X-RAY DIFFRACTION	99.96
7.59-54.27	0.1546	120	0.1362	2716	X-RAY DIFFRACTION	97.66

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	6.50570844418	2.71084631693	4.64802221753	3.83904389047	5.74331562667	8.66877474991	-0.341901940894	-1.34730501025	-0.00263544468299	1.83566158317	-0.357371743113	1.18321848765	1.05837323365	-0.732915977186	0.736409206926	0.638800679465	0.0706048951609	0.198162856399	0.32898665474	-0.0748623359739	0.387448391891	13.2369802626	-40.984522012	18.978946111
2	0.588387394556	-0.23619007974	0.0613266678253	1.46146000896	0.0467740061433	0.493725848213	0.165358141182	0.138668136039	-0.112935529967	0.0152385882875	-0.0629765133712	-0.0779531378941	0.42866584017	0.178424015182	-0.0757923942445	0.347576018527	0.0972314666615	-0.0352722462667	0.180110605878	-0.0320667886877	0.173825333668	17.2387602264	-54.0423516088	8.19584496931
3	3.28100538786	5.1955384271	2.51510548145	8.24239224525	3.9972009502	1.94294561171	0.2360912651	-0.363444038204	-0.0879121546273	0.608081047228	-0.162381554385	0.167011597564	0.246107491143	-0.362775629479	-0.0387881905421	0.131656899697	0.0220122436195	0.0764996377062	0.142356471188	0.0308181434331	0.305509485669	6.13232328645	-42.1840459096	10.0723144682
4	0.0750618204649	0.371581069396	0.213084842393	3.77317985786	0.901264957516	1.79885671997	-0.0108842121396	-0.0348025776192	0.00261311994268	-0.431939128406	-0.121302370778	-0.0896019243182	-0.403838842056	-0.100258151445	0.14050070607	0.0429029822107	0.0362816965945	-0.0346964383139	0.165563555105	0.028852336393	0.184854387751	8.43891505451	-25.2903812076	8.78946623655
5	0.679614954428	-0.666946503815	-0.376483620522	1.36442994981	0.871422014733	2.10052535382	-0.287885946104	-0.183041566754	0.0674792836513	0.228404364522	0.0671801239617	0.141257212205	-0.201755662478	-0.329312485475	0.172393898	0.150304873662	0.079493540736	-0.0136680572668	0.279324022514	-0.0518357807152	0.264843124513	2.11784885244	-19.2820277779	21.8956219684
6	2.35186183971	2.15915697375	0.545212664933	4.87807559294	-3.58435888099	6.80223742755	-0.102428217199	0.126131149149	0.0146452178394	0.0937574897517	-0.188490040198	0.279361252875	-0.191436141043	-0.42088033443	0.192896254005	0.0626506612136	0.0676738291091	-0.0399143254275	0.15395974106	-0.0326623948967	0.223102993081	-0.146231959879	-23.8008944179	11.4960592094
7	7.1531608159	6.79711767984	-5.36637124605	7.40231017933	-4.97039287269	4.04338538362	0.20172505695	-1.53591163073	-0.967642636577	1.88683686082	-0.471241288542	-0.390498220164	0.251259299825	0.19229035675	0.28035097799	0.644488565944	0.107520913205	0.0302765866599	0.582669548991	0.124017038348	0.355002392959	27.8124495617	-30.4134821097	25.9504851498
8	0.40950929105	0.159103646814	0.383506410777	1.17796042104	0.210240148932	0.716110802017	-0.264353050749	0.167111691799	0.195515992232	0.0810384068648	-0.0810835719675	-0.0341122789093	-0.502835688765	0.398400341008	0.138021215198	0.400072278161	-0.153572107929	-0.0908307683755	0.241585118413	0.102877510555	0.290406603725	28.2866692335	-13.0289708287	20.0995157148
9	2.01354139456	1.18687353973	-0.412438436983	3.79194004679	0.867420483653	0.604084494921	0.0447880238568	-0.172866620255	-0.159680988766	-0.0787848403324	-0.21341528588	-0.444788350058	-0.212037278051	0.460024543798	0.110775143786	0.178324364609	-0.09336196479	0.0437443403926	0.601803374804	0.121594239343	0.224531235262	40.5287719308	-23.844971435	16.5326341803
10	0.58438614578	-0.272512821401	-0.00809423470355	0.654848596589	0.0636443838658	0.206410212607	0.027308788631	0.0454142236985	0.0324432086879	0.0772407298578	0.0124186851882	-0.0769212071091	0.231274561448	0.399156065577	-0.0820955936693	0.227183759088	0.24883933294	0.0391053279016	0.497624686222	-0.0375275055659	0.230343569581	38.0532116076	-52.2296089851	22.1642409161
11	7.89149741524	-1.65613559333	-0.904049003966	2.448897373	0.748128875491	0.809590783138	-0.0918877974783	-0.140858571971	-0.0667285890448	0.0895087015856	0.0259842837173	-0.00098384999024	0.0536861825178	0.150612820442	0.0488544726558	0.113987644754	0.114862051016	0.0250351252154	0.447873614821	0.0127282753625	0.213253013741	45.1412414103	-43.8383919525	19.2850114614
12	6.6591055641	0.672380371589	1.81942542957	4.21869449499	4.991091857	6.92766355557	0.11912082387	0.352977749616	2.15037922349	-0.607931489758	0.136052446271	0.121034109159	-2.24269898558	0.479286691848	-0.245371158864	0.617590839726	-0.00416467062259	0.0688602490159	0.317086234729	0.0121649962325	0.496432122746	21.801173377	-65.1845799088	46.8828442211
13	0.366696414309	0.108674802092	0.229002907209	0.771222704188	0.389975459702	0.280270807599	0.30402417761	0.0437099041096	-0.226846595757	-0.0977256648559	-0.0962975936039	-0.0257162705325	0.631362062693	0.0355101588494	0.0470162952106	0.732610516308	0.178203878095	-0.119777075857	0.189035699546	-0.0399371887571	0.278551252242	22.855982677	-77.0679310312	35.6299830695
14	7.81211025169	-4.6532395839	-7.42994468212	2.77690038062	4.41623890678	7.08193135935	0.133446101538	-0.1362871799	0.275094341778	-0.625262464281	0.204963318731	-0.363253001571	-0.0814720775463	0.352105923265	-0.345589719356	0.556125169537	0.062710524498	-0.0137075469522	0.29338806254	-0.0341253795143	0.18682461351	31.1516054538	-71.7033411631	48.602197213
15	0.707260361165	0.00533463932111	-0.242376524346	1.16126177804	-0.372775428279	0.550960670463	0.152730735783	-0.128221460094	-0.115046362782	0.170190264479	-0.0247263680785	-0.0150910880364	0.668401258013	0.083379124443	-0.115274044463	0.416349233796	0.00890624026929	-0.0913165263796	0.30585578209	0.0977571857813	0.196243539533	24.2642319306	-71.0059690451	65.6703954758
16	8.43667721113	-5.43499956847	1.18171952026	7.4264197881	-3.65550104122	2.29983029732	-0.14697765747	-1.48997623616	1.52121280504	1.30643819648	0.0980493417875	-1.33559152469	-0.95771863944	0.44654600689	-0.0127411770956	0.656370509331	-0.085626883929	-0.0732166415293	0.459216641017	-0.119617311229	0.382469568193	28.1245778488	-41.5804162425	79.8767390673
17	1.40528985062	-1.08882199299	1.11254064818	2.66086194704	0.857151300953	2.75145820634	0.00421291805949	-0.0250061624949	0.278975426607	-0.134367712998	0.157170997171	-0.307818511115	-0.50547801813	0.10268430211	-0.146522048027	0.308374682328	-0.0702622555851	0.0657310868346	0.208811419559	-0.00377056215629	0.237374945491	27.4583354558	-55.4036289769	67.3750278634
18	5.2963288459	2.7755994926	-3.28117048362	7.25696353827	-4.13026828786	5.23243853392	0.204394148584	-0.424402795666	-0.156921174335	0.0380154308519	-0.0528859621525	-0.12554591157	0.631317378162	-0.0184898798653	-0.133899589003	0.336906261399	-0.0275402738596	-0.105662394451	0.345925732269	0.0356881824434	0.130383450186	27.7595269876	-71.1228640962	70.8761376125
19	2.36764451129	0.648805768221	-0.407914896431	0.849186263016	-2.4405248074	8.67206998289	-0.0286113420476	-0.0833110501062	-0.0583500440807	-0.198804986732	-0.0548596555349	-0.134352687154	0.776708076639	-0.218301989591	0.117083086191	0.457854901591	0.154887850135	0.0295415535511	0.354004176635	-0.0868816550711	0.231769927779	16.172009196	-22.6094451104	62.9908624032
20	0.044849097935	-0.0318548516235	-0.170251488059	0.351608759498	-0.270350877282	1.07892038591	0.0103528343828	-0.0797413491171	0.0972953817502	-0.00658201314126	-0.089861665242	0.180717025686	-0.279621901558	-0.226860007855	0.0511691817505	0.504559451294	0.21123956726	-0.042804494935	0.439092996836	-0.179950013147	0.296015617344	13.7160456247	-14.5199198891	71.1582803398
21	0.431821409675	-0.409756458555	0.147025327821	1.11850678465	0.367559772895	1.46684399156	-0.0887749486043	-0.161690349369	-0.0388425902754	0.0827967870219	0.0155291484494	0.0812210684073	0.310044725074	-0.612387116362	0.126013171645	0.371119888878	0.0912751742386	0.0677680804175	0.629989937802	-0.088766983424	0.19324195416	10.9340467565	-36.2096136616	85.7749155738
22	2.01524108143	-0.0281150521083	-0.225991592484	3.35382428908	-3.20875316994	3.44218215332	-0.171841246587	-0.0995852074448	0.111201381086	0.199266684215	-0.105974391434	-0.240586640488	-0.320529317721	-0.139200236083	0.265464740903	0.381352582538	0.137580808319	-0.0539364848687	0.355899807149	-0.11276485206	0.236261411763	24.2031755157	-20.0810549125	78.3047948792
23	1.85263473704	0.0827211447368	-1.88253966457	0.84076487468	0.484859377117	5.2255417718	-0.365554211084	-0.186946953534	0.163164593746	0.0551862063303	-0.0160246745189	-0.101243818316	-0.472820867335	0.395807081741	0.307055891205	0.290502833862	0.000730656152267	-0.125067758614	0.219206003524	0.0167594659539	0.25065519459	31.4951474411	-12.700525453	50.390264506
24	4.73965996738	-2.33745530739	-2.07638776478	6.05248228928	1.82497920117	2.4848753542	-0.00871804138038	-0.0411345024777	0.689769592705	0.0243633511969	-0.0918160694097	-0.0604626536645	-0.630186200089	-0.370765859676	0.0761378819706	0.5991914702	0.085199455801	-0.181478947258	0.31763349088	-0.0539065021124	0.320141969888	30.5378553519	-5.44888381345	63.8871310888
25	1.14024202755	0.273571208336	-1.6345484844	0.730725103196	-0.644661351705	2.43987515564	0.151173857473	-0.29539034251	-0.134279553797	-0.0444241694281	0.0739777558156	0.0311400098772	0.0959942377978	-0.131852439298	-0.142036929977	0.475626340601	-0.212476924848	-0.0704141207134	0.360727153064	0.11594825254	0.243459189459	5.73840817993	-71.0275536008	54.4213952448
26	0.609890313568	-0.227603282471	-0.019180041243	1.2701516508	-0.288601163339	0.878248103036	0.121769463871	-0.351087033949	0.0112414170696	0.276936080463	0.0757486130592	0.108301173989	-0.164740702475	-0.313722797866	-0.152951582399	0.404673962153	-0.186917239475	0.0186099341498	0.644009739998	0.159781273245	0.217833754343	-1.56778415066	-60.4246694767	77.1197307887
27	6.66573180877	-1.93984596548	-6.07358370176	1.45472997851	1.7107753532	8.90366895392	0.437675675774	0.193536976576	0.342580855088	0.00542459728409	-0.0698868550604	-0.00810122328279	-0.331059909595	-0.560985373542	-0.44380256478	0.449216081767	-0.284620809752	-0.0603284873124	0.624063431164	0.157214790883	0.269670213463	-7.13418759694	-64.8355075435	56.9650740421
28	0.275745377291	-0.0952746016007	0.0735268586089	0.538618191219	0.357911788535	0.310528136029	0.130063686776	-0.217180262604	-0.220695206991	0.00236213514114	-0.0436540997305	0.0859656398378	0.396375801726	-0.233401901972	-0.0169005858745	0.569061922505	-0.224988808596	-0.163758595808	0.303014832607	0.11545163754	0.34825901407	-1.37948520883	-75.3814558459	42.8622801102
29	0.408607836297	0.689421087811	-0.653443369901	1.64163833072	-1.43116060739	1.28652981689	0.266894925796	-0.107620548103	-0.12129920179	-0.00929057813205	0.0538324179616	0.229382371288	0.340774528538	-0.278736251996	-0.317574783528	0.466125047377	-0.130477349651	-0.105138054887	0.233784830096	0.0595040010302	0.267321418749	-1.15692002002	-65.4672752779	30.8936605948
30	4.02543583789	2.06922013713	-0.852041610781	2.50115168049	-1.43664172505	1.30509712978	0.242798721652	-0.204894949867	-0.211903633061	0.166420561747	0.0271413699851	0.249193632438	0.0841308955443	-0.0351460576331	-0.225922690226	0.492036490308	-0.264831868384	-0.144838345833	0.389937677313	0.102958303468	0.330173140979	-8.0716922419	-71.1043985853	38.2572182409
31	6.22058870285	1.72523165199	1.71695571914	1.54228297481	-2.18720445843	7.14138829397	0.244396154984	0.71304670429	-1.4540040448	-0.393057441288	-0.0228299657545	-0.0174522179035	1.20927440737	0.143999964123	-0.216797791289	0.603808248123	0.282083475166	-0.146566628703	0.550165379578	-0.155832020897	0.450378528298	4.79062377026	-17.7018521068	52.5547064278
32	0.162446602132	0.0670883254343	0.0585044967188	0.333708920087	0.022894848793	0.61599316597	-0.310712003021	-0.0680809666695	0.173517044431	-0.134281468362	-0.0424895289007	0.139653823079	-0.476783887392	-0.385372717487	0.0805225047644	0.725159660583	0.288782752934	-0.129813322598	0.435402213832	-0.165633340164	0.336098122752	5.98112575005	-5.17089510275	55.2274075229
33	0.489534144903	-0.203946996322	-0.101853988526	0.55296394918	0.1472243162	0.50519664009	-0.238615989079	-0.14449459776	0.240771593206	0.0174301858212	-0.00960768154827	-0.0308238071853	-0.467821926301	-0.144318102522	-0.120135297736	0.723261995815	0.255607465771	-0.213001189784	0.247308766477	-0.160717920156	0.358624531042	8.87369250705	-0.382524831647	42.919172132
34	0.47161467158	-0.135678760013	-0.0091058706786	0.650992664569	-0.502664869799	1.46756097473	-0.08614474742	-0.0864627072338	-0.0285540081822	0.168473259418	-0.0121132430377	0.154172553484	-0.021127391942	-0.147840852586	0.0970997434119	0.527824542769	0.3645792796	-0.0821472909347	0.672346568449	-0.220254456728	0.312063273337	-6.11815913937	-6.83354852316	56.2230940994
35	0.468517251774	-0.526052634523	-0.449767953565	1.89294475733	0.326457157202	1.43291134318	-0.110538499481	-0.211726747424	-0.0983218819369	0.0170842588274	0.0603946909333	0.230421756746	0.0478295732508	-0.693384450837	0.144815294965	0.328847094203	0.127722265609	0.0562480269203	0.837664359209	-0.0144873952984	0.20336458936	-9.26604100133	-34.9488501213	65.9470510555
36	1.89240320758	-0.641582724143	-0.356369708328	0.940141046254	0.368022565257	0.931443049015	0.0276661050612	-0.082022578559	0.187996823747	0.0439735244625	0.0084316483331	0.00666971763922	-0.0779724700421	-0.120278931221	0.0569920754364	0.3716219964	0.320625784735	0.0311233788625	0.802820233476	-0.107532963302	0.35143032316	-12.6570093661	-20.9678052731	71.0968100952

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid -2 through 4 )	A	A	-2 - 4	1 - 7
2	2	chain 'A' and (resid 5 through 163 )	A	A	5 - 163	8 - 166
3	3	chain 'D' and (resid 1 through 15 )	D	B	1 - 15	1 - 15
4	4	chain 'D' and (resid 16 through 63 )	D	B	16 - 63	16 - 63
5	5	chain 'D' and (resid 64 through 154 )	D	B	64 - 154	64 - 154
6	6	chain 'D' and (resid 155 through 173 )	D	B	155 - 173	155 - 173
7	7	chain 'B' and (resid -3 through 4 )	B	C	-3 - 4	1 - 8
8	8	chain 'B' and (resid 5 through 163 )	B	C	5 - 163	9 - 167
9	9	chain 'C' and (resid 1 through 34 )	C	D	1 - 34	1 - 34
10	10	chain 'C' and (resid 35 through 154 )	C	D	35 - 154	35 - 154
11	11	chain 'C' and (resid 155 through 173 )	C	D	155 - 173	155 - 173
12	12	chain 'E' and (resid -1 through 4 )	E	E	-1 - 4	1 - 6
13	13	chain 'E' and (resid 5 through 163 )	E	E	5 - 163	7 - 165
14	14	chain 'F' and (resid 1 through 16 )	F	F	1 - 16	1 - 16
15	15	chain 'F' and (resid 17 through 63 )	F	F	17 - 63	17 - 63
16	16	chain 'F' and (resid 64 through 76 )	F	F	64 - 76	64 - 76
17	17	chain 'F' and (resid 77 through 143 )	F	F	77 - 143	77 - 143
18	18	chain 'F' and (resid 144 through 173 )	F	F	144 - 173	144 - 173
19	19	chain 'G' and (resid 0 through 15 )	G	G	0 - 15	1 - 16
20	20	chain 'G' and (resid 16 through 47 )	G	G	16 - 47	17 - 48
21	21	chain 'G' and (resid 48 through 163 )	G	G	48 - 163	49 - 164
22	22	chain 'H' and (resid 1 through 34 )	H	H	1 - 34	1 - 34
23	23	chain 'H' and (resid 35 through 143 )	H	H	35 - 143	35 - 143
24	24	chain 'H' and (resid 144 through 173 )	H	H	144 - 173	144 - 173
25	25	chain 'I' and (resid 1 through 47 )	I	I	1 - 47	1 - 47
26	26	chain 'I' and (resid 48 through 163 )	I	I	48 - 163	48 - 163
27	27	chain 'J' and (resid 1 through 16 )	J	J	1 - 16	1 - 16
28	28	chain 'J' and (resid 17 through 63 )	J	J	17 - 63	17 - 63
29	29	chain 'J' and (resid 64 through 154 )	J	J	64 - 154	64 - 154
30	30	chain 'J' and (resid 155 through 173 )	J	J	155 - 173	155 - 173
31	31	chain 'K' and (resid -1 through 4 )	K	K	-1 - 4	1 - 6
32	32	chain 'K' and (resid 5 through 68 )	K	K	5 - 68	7 - 70
33	33	chain 'K' and (resid 69 through 163 )	K	K	69 - 163	71 - 165
34	34	chain 'L' and (resid 1 through 34 )	L	L	1 - 34	1 - 34
35	35	chain 'L' and (resid 35 through 143 )	L	L	35 - 143	35 - 143
36	36	chain 'L' and (resid 144 through 173 )	L	L	144 - 173	144 - 173

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