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- PDB-216d: CRYSTAL STRUCTURES OF THE B-FORM DNA-RNA CHIMER (5'-D(*IP*)-R(*CP... -
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Open data
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Basic information
Entry | Database: PDB / ID: 216d | ||||||||||||||||||
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Title | CRYSTAL STRUCTURES OF THE B-FORM DNA-RNA CHIMER (5'-D(*IP*)-R(*CP*)-D(*IP*CP*IP*CP*IP*C)-3') COMPLEXED WITH DISTAMYCIN | ||||||||||||||||||
![]() | DNA/RNA (5'-D(*![]() DNA-RNA HYBRID / B-DNA/RNA / DOUBLE HELIX / COMPLEXED WITH DRUG / MODIFIED | Function / homology | DISTAMYCIN A / DNA/RNA hybrid | ![]() Method | ![]() ![]() Chen, X. / Ramakrishnan, B. / Sundaralingam, M. | ![]() ![]() Title: Crystal structures of B-form DNA-RNA chimers complexed with distamycin. Authors: Chen, X. / Ramakrishnan, B. / Sundaralingam, M. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 14 KB | Display | ![]() |
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PDB format | ![]() | 9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: DNA/RNA hybrid | Mass: 2384.537 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: Chemical | ChemComp-DMY / |
#3: Chemical | ChemComp-MG / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.38 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 7 / Details: pH 7.00, VAPOR DIFFUSION, HANGING DROP / Temp details: ROOM TEMPERATURE | |||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS pH: 7 | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Highest resolution: 1.73 Å / Num. all: 10170 / Num. obs: 2556 / Observed criterion σ(F): 2 |
Reflection | *PLUS Highest resolution: 1.73 Å / % possible obs: 95 % / Observed criterion σ(F): 2 / Num. measured all: 10170 |
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Processing
Software | Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.73→8 Å / σ(F): 2
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 1.73→8 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.73 Å / Lowest resolution: 8 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |