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- PDB-1z7p: Solution structure of reduced glutaredoxin C1 from Populus tremul... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1z7p | ||||||
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Title | Solution structure of reduced glutaredoxin C1 from Populus tremula x tremuloides | ||||||
![]() | glutaredoxin | ||||||
![]() | ELECTRON TRANSPORT | ||||||
Function / homology | ![]() glutathione disulfide oxidoreductase activity / 2 iron, 2 sulfur cluster binding / cellular response to oxidative stress / identical protein binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics simulated annealing | ||||||
![]() | Feng, Y. / Zhong, N. / Rouhier, N. / Jacquot, J.P. / Xia, B. | ||||||
![]() | ![]() Title: Structural Insight into Poplar Glutaredoxin C1 with a Bridging Iron-Sulfur Cluster at the Active Site Authors: Feng, Y. / Zhong, N. / Rouhier, N. / Hase, T. / Kusunoki, M. / Jacquot, J.P. / Jin, C. / Xia, B. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 684.9 KB | Display | ![]() |
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PDB format | ![]() | 573.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1z7rC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 12529.306 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: pET3d / Species (production host): Escherichia coli / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1.1mM uniformly 13C/15N-labeled protein, 40mM potassium phosphate buffer, 90% H2O/10% D2O, 40mM DTT, 0.01% NaN3 and 0.01% DSS, protease inhibitor cocktail Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 40mM potassium phosphate / pH: 6.4 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: molecular dynamics simulated annealing / Software ordinal: 1 Details: CYS 31 is present as the thiolate with a negative charge on the sulfur. | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |