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Yorodumi- PDB-1ytm: Crystal structure of phosphoenolpyruvate carboxykinase of Anaerob... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ytm | ||||||
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Title | Crystal structure of phosphoenolpyruvate carboxykinase of Anaerobiospirillum succiniciproducens complexed with ATP, oxalate, magnesium and manganese ions | ||||||
Components | phosphoenolpyruvate carboxykinase [ATP] | ||||||
Keywords | LYASE / kinase / domain closure / nucleotide binding / parallel beta sheet-like hydrogen bond | ||||||
Function / homology | Function and homology information phosphoenolpyruvate carboxykinase (ATP) / phosphoenolpyruvate carboxykinase (ATP) activity / gluconeogenesis / ATP binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Anaerobiospirillum succiniciproducens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Delbaere, L.T.J. / Cotelesage, J.J.H. | ||||||
Citation | Journal: Int.J.Biochem.Cell Biol. / Year: 2005 Title: Crystal structure of Anaerobiospirillum succiniciproducens PEP carboxykinase reveals an important active site loop Authors: Cotelesage, J.J.H. / Prasad, L. / Zeikus, J.G. / Laivenieks, M. / Delbaere, L.T.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ytm.cif.gz | 222.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ytm.ent.gz | 173.7 KB | Display | PDB format |
PDBx/mmJSON format | 1ytm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yt/1ytm ftp://data.pdbj.org/pub/pdb/validation_reports/yt/1ytm | HTTPS FTP |
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-Related structure data
Related structure data | 1yvyC 1aq2S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 58709.578 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Anaerobiospirillum succiniciproducens (bacteria) Production host: Escherichia coli (E. coli) References: UniProt: O09460, phosphoenolpyruvate carboxykinase (ATP) |
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-Non-polymers , 5 types, 392 molecules
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.5 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: MES, DTT, sodium citrate, PEG 4000, 2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 105 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 14-ID-B / Wavelength: 0.9 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 30, 2003 |
Radiation | Monochromator: Diamond (111) double-crystal monochromator Bent cylindrical Si-mirror (Rh coating) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 60877 / % possible obs: 99.8 % / Observed criterion σ(I): 5 / Redundancy: 7.1 % / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 4.4 % / Mean I/σ(I) obs: 2.4 / Num. unique all: 5921 / Rsym value: 0.38 / % possible all: 98.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1AQ2 Resolution: 2.2→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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Refine LS restraints |
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