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Yorodumi- PDB-1yfi: Crystal Structure of restriction endonuclease MspI in complex wit... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1yfi | ||||||
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Title | Crystal Structure of restriction endonuclease MspI in complex with its cognate DNA in P212121 space group | ||||||
Components |
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Keywords | Hydrolase/DNA / protein-DNA complex / Hydrolase-DNA COMPLEX | ||||||
Function / homology | Restriction endonuclease, type II, MspI / Restriction endonuclease MspI / type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / Restriction endonuclease type II-like / DNA binding / DNA / Type II restriction enzyme MspI Function and homology information | ||||||
Biological species | Moraxella sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Xu, Q.S. / Kucera, R.B. / Roberts, R.J. / Guo, H.-C. | ||||||
Citation | Journal: Protein Sci. / Year: 2005 Title: Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure. Authors: Xu, Q.S. / Roberts, R.J. / Guo, H.-C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1yfi.cif.gz | 136.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1yfi.ent.gz | 104.5 KB | Display | PDB format |
PDBx/mmJSON format | 1yfi.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yf/1yfi ftp://data.pdbj.org/pub/pdb/validation_reports/yf/1yfi | HTTPS FTP |
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-Related structure data
Related structure data | 1sa3S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3046.980 Da / Num. of mol.: 4 / Source method: obtained synthetically #2: Protein | Mass: 29876.010 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Moraxella sp. (bacteria) / Gene: mspIR / Plasmid: PCAD39 / Production host: Escherichia coli (E. coli) / Strain (production host): ER2502 References: UniProt: P11405, type II site-specific deoxyribonuclease #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 52 % | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: MES, ammonium sulfate, calcium chloride, PEG 8000, glycerol , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.904 Å |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Apr 8, 2001 Details: DOUBLE-CRYSTAL MONOCHROMATOR SI(111), BEAM FOCUSED BY A TOROIDAL MIRROR |
Radiation | Monochromator: DOUBLE-CRYSTAL MONOCHROMATOR Si(111) MIRROR + NI FILTER Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.904 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→100 Å / Num. all: 21426 / Num. obs: 21426 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 10 % / Biso Wilson estimate: 27.9 Å2 / Rmerge(I) obs: 0.072 / Rsym value: 0.072 / Net I/σ(I): 28.7 |
Reflection shell | Resolution: 2.7→2.8 Å / Rmerge(I) obs: 0.282 / Mean I/σ(I) obs: 7 / Num. unique all: 2100 / Rsym value: 0.282 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1SA3 Resolution: 2.7→46.94 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1435032.38 / Data cutoff high rms absF: 1435032.38 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 10 Å2 / ksol: 0.318155 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→46.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.7→2.8 Å / Rfactor Rfree error: 0.027 / Total num. of bins used: 10
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Xplor file |
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