分子量: 2691.264 Da / 分子数: 1 / 由来タイプ: 合成 詳細: Humanin was prepared by Fmoc-solid phase synthesis on -Cl-trityl-amidomethyl polystyrene resin. The peptide was purified to 95% with semi-preparative RP-HPLC and suitably characterized. ...詳細: Humanin was prepared by Fmoc-solid phase synthesis on -Cl-trityl-amidomethyl polystyrene resin. The peptide was purified to 95% with semi-preparative RP-HPLC and suitably characterized. Evangelou, A., Zikos, C., Livaniou, E., and Evangelatos, G.P. (2004) High-yield, solid-phase synthesis of humanin, an Alzheimer s disease associated, novel 24-mer peptide which contains a difficult sequence, J. Peptide Science 10, 631-635. 参照: UniProt: Q8IVG9
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
1
1
1
2D NOESY
1
2
1
2D TOCSY
2
3
1
2D NOESY
2
4
1
2D TOCSY
NMR実験の詳細
Text: This structure was determined using standard 2D homonuclear techniques.
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試料調製
詳細
内容: NMR studies of humanin were carried out in H2O/TFE-d3 7:3 solution containing 60 microM NaN3 at 280 and 298 K. The concentration of the sample was 1.7 mM and the pH of the solution was 2.7 ...内容: NMR studies of humanin were carried out in H2O/TFE-d3 7:3 solution containing 60 microM NaN3 at 280 and 298 K. The concentration of the sample was 1.7 mM and the pH of the solution was 2.7 (uncorrected for the presence of TFE). 溶媒系: H2O/TFE-d3 7:3 solution containing 60 microM NaN3
試料状態
Conditions-ID
イオン強度
pH
圧 (kPa)
温度 (K)
1
60microMsodiumazide
2.7
ambient
298K
2
60microMsodiumazide
2.7
ambient
280K
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ: Bruker AVANCE / 製造業者: Bruker / モデル: AVANCE / 磁場強度: 500 MHz