negative regulation of interleukin-18 production / receptor antagonist activity / negative regulation of neuroinflammatory response / negative regulation of interleukin-1 production / negative regulation of NLRP3 inflammasome complex assembly / negative regulation of execution phase of apoptosis / negative regulation of amyloid fibril formation / Formyl peptide receptors bind formyl peptides and many other ligands / leukocyte chemotaxis / negative regulation of response to oxidative stress ...negative regulation of interleukin-18 production / receptor antagonist activity / negative regulation of neuroinflammatory response / negative regulation of interleukin-1 production / negative regulation of NLRP3 inflammasome complex assembly / negative regulation of execution phase of apoptosis / negative regulation of amyloid fibril formation / Formyl peptide receptors bind formyl peptides and many other ligands / leukocyte chemotaxis / negative regulation of response to oxidative stress / sperm flagellum / supramolecular fiber organization / sperm midpiece / mitochondrion organization / G protein-coupled receptor binding / negative regulation of inflammatory response / cellular response to amyloid-beta / cell-cell signaling / G alpha (i) signalling events / G alpha (q) signalling events / intracellular iron ion homeostasis / negative regulation of neuron apoptotic process / signaling receptor binding / negative regulation of apoptotic process / apoptotic process / perinuclear region of cytoplasm / mitochondrion / extracellular space / extracellular region / identical protein binding / nucleus / cytoplasm Similarity search - Function
Mass: 2691.264 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: Humanin was prepared by Fmoc-solid phase synthesis on -Cl-trityl-amidomethyl polystyrene resin. The peptide was purified to 95% with semi-preparative RP-HPLC and suitably characterized. ...Details: Humanin was prepared by Fmoc-solid phase synthesis on -Cl-trityl-amidomethyl polystyrene resin. The peptide was purified to 95% with semi-preparative RP-HPLC and suitably characterized. Evangelou, A., Zikos, C., Livaniou, E., and Evangelatos, G.P. (2004) High-yield, solid-phase synthesis of humanin, an Alzheimer s disease associated, novel 24-mer peptide which contains a difficult sequence, J. Peptide Science 10, 631-635. References: UniProt: Q8IVG9
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D NOESY
1
2
1
2D TOCSY
2
3
1
2D NOESY
2
4
1
2D TOCSY
NMR details
Text: This structure was determined using standard 2D homonuclear techniques.
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Sample preparation
Details
Contents: NMR studies of humanin were carried out in H2O/TFE-d3 7:3 solution containing 60 microM NaN3 at 280 and 298 K. The concentration of the sample was 1.7 mM and the pH of the solution was 2.7 ...Contents: NMR studies of humanin were carried out in H2O/TFE-d3 7:3 solution containing 60 microM NaN3 at 280 and 298 K. The concentration of the sample was 1.7 mM and the pH of the solution was 2.7 (uncorrected for the presence of TFE). Solvent system: H2O/TFE-d3 7:3 solution containing 60 microM NaN3
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
60microMsodiumazide
2.7
ambient
298K
2
60microMsodiumazide
2.7
ambient
280K
-
NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 500 MHz
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