- PDB-1xsf: Solution structure of a resuscitation promoting factor domain fro... -
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Basic information
Entry
Database: PDB / ID: 1xsf
Title
Solution structure of a resuscitation promoting factor domain from Mycobacterium tuberculosis
Components
Probable resuscitation-promoting factor rpfB
Keywords
CELL CYCLE / HYDROLASE / LYSOZYME-LIKE STRUCTURE
Function / homology
Function and homology information
dormancy exit of symbiont in host / positive regulation of growth rate / Hydrolases / quorum sensing / regulation of cell population proliferation / hydrolase activity / negative regulation of gene expression / positive regulation of gene expression / extracellular region / plasma membrane Similarity search - Function
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelength
Relative weight: 1
NMR spectrometer
Type
Manufacturer
Model
Field strength (MHz)
Spectrometer-ID
Bruker AVANCE
Bruker
AVANCE
500
1
Bruker AVANCE
Bruker
AVANCE
600
2
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Processing
NMR software
Name
Version
Developer
Classification
Gifa
4.4
Pons
processing
NMRView
5.0.4
Johnson
dataanalysis
TALOS
3
Cornilescu
dataanalysis
ARIA
1.2
Linge
structuresolution
CNS
1.1
Brunger
refinement
Refinement
Method: molecular dynamics in explicit solvent / Software ordinal: 1 Details: the structures are based on a total of 1757 restraints, 1613 are NOE-derived distance constraints, 124 dihedral angle restraints, 20 distance restraints from hydrogen bonds.
NMR representative
Selection criteria: lowest energy
NMR ensemble
Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 30 / Conformers submitted total number: 30
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