+Open data
-Basic information
Entry | Database: PDB / ID: 1xbs | ||||||
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Title | Crystal structure of human dim2: a dim1-like protein | ||||||
Components | Dim1-like protein | ||||||
Keywords | TRANSCRIPTION / CELL CYCLE / SPLICEOSOMAL PROTEIN / THIOREDOXIN / SNRNP | ||||||
Function / homology | Function and homology information U5 snRNP / U4/U6 x U5 tri-snRNP complex / spliceosomal complex / mRNA splicing, via spliceosome / cell cycle / nucleoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Simeoni, F. / Arvai, A. / Hopfner, K.-P. / Bello, P. / Gondeau, C. / Heitz, F. / Tainer, J. / Divita, G. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Biochemical Characterization and Crystal Structure of a Dim1 Family Associated Protein: Dim2 Authors: Simeoni, F. / Arvai, A. / Bello, P. / Gondeau, C. / Hopfner, K.-P. / Neyroz, P. / Heitz, F. / Tainer, J. / Divita, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xbs.cif.gz | 38.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xbs.ent.gz | 26.9 KB | Display | PDB format |
PDBx/mmJSON format | 1xbs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xb/1xbs ftp://data.pdbj.org/pub/pdb/validation_reports/xb/1xbs | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 17034.611 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pGEX4T / Production host: Escherichia coli (E. coli) / Strain (production host): DH5a / References: GenBank: 45594298, UniProt: Q9NX01*PLUS |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.98 Å3/Da / Density % sol: 58.4 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: Ammonium sulfate, sodium chloride, EDTA, Tris-HCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 200 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL7-1 / Wavelength: 1.08 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 23, 2000 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→30 Å / Num. all: 6836 / Num. obs: 6538 / % possible obs: 89.9 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
Reflection shell | Highest resolution: 2.5 Å / Num. unique all: 615 / % possible all: 93.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.5→30 Å / σ(F): -3 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.5→30 Å
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Refine LS restraints |
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