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Yorodumi- PDB-1wez: Solution structure of RRM domain in heterogeneous nuclear ribonuc... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wez | ||||||
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Title | Solution structure of RRM domain in heterogeneous nuclear ribonucleoprotein H' | ||||||
Components | Heterogeneous nuclear ribonucleoprotein H' | ||||||
Keywords | RNA BINDING PROTEIN / structural genomics / RRM domain / heterogeneous nuclear ribonucleoprotein H' / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information regulation of RNA splicing / Processing of Capped Intron-Containing Pre-mRNA / mRNA Splicing - Major Pathway / postsynaptic density / ribonucleoprotein complex / RNA binding / nucleoplasm / membrane / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | He, F. / Muto, Y. / Inoue, M. / Kigawa, T. / Shirouzu, M. / Terada, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of RRM domain in heterogeneous nuclear ribonucleoprotein H' Authors: He, F. / Muto, Y. / Inoue, M. / Kigawa, T. / Shirouzu, M. / Terada, T. / Yokoyama, S. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR DETERMINED |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wez.cif.gz | 571.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wez.ent.gz | 476.5 KB | Display | PDB format |
PDBx/mmJSON format | 1wez.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wez_validation.pdf.gz | 345.2 KB | Display | wwPDB validaton report |
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Full document | 1wez_full_validation.pdf.gz | 508.1 KB | Display | |
Data in XML | 1wez_validation.xml.gz | 39.1 KB | Display | |
Data in CIF | 1wez_validation.cif.gz | 59.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/we/1wez ftp://data.pdbj.org/pub/pdb/validation_reports/we/1wez | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 10819.810 Da / Num. of mol.: 1 / Fragment: RRM domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell free protein synthesis / Gene: CBL00922 / Plasmid: P040223-29 / References: UniProt: P55795 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.8mM U-15,13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |