- PDB-1up0: Structure of the endoglucanase Cel6 from Mycobacterium tuberculos... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 1up0
Title
Structure of the endoglucanase Cel6 from Mycobacterium tuberculosis in complex with cellobiose at 1.75 angstrom
Components
PUTATIVE CELLULASE CEL6
Keywords
HYDROLASE / GLYCOSIDE HYDROLASE / FAMILY 6
Function / homology
Function and homology information
Hydrolases; Glycosylases; Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds / cellulase activity / cellulose catabolic process / extracellular region / membrane Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 317 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
ONLY THE CATALYTIC DOMAIN HAS BEEN CLONED CORRESPONDING TO RESIDUES 88 TO 380.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.03 Å3/Da / Density % sol: 38.81 %
Crystal grow
pH: 4.6 Details: 10 % PEG 4K, 100 MM NA ACETATE PH 4.6, 200 MM LISO4. THE PROTEIN WAS AT 10MG/ML AND INCUBATED WITH 1 MM OF FLUORESCEINYL DERIVED 1-BETA-METHYL-CELLOTRIOSIDE-THIO-GLUCOSIDE FOR 1 HOUR PRIOR ...Details: 10 % PEG 4K, 100 MM NA ACETATE PH 4.6, 200 MM LISO4. THE PROTEIN WAS AT 10MG/ML AND INCUBATED WITH 1 MM OF FLUORESCEINYL DERIVED 1-BETA-METHYL-CELLOTRIOSIDE-THIO-GLUCOSIDE FOR 1 HOUR PRIOR CRYSTALLISATION. 30% PEG400 WAS ADDED AS CRYOPROTECTANT