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- PDB-1ufh: Structure of putative acetyltransferase, YYCN protein of Bacillus... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ufh | ||||||
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Title | Structure of putative acetyltransferase, YYCN protein of Bacillus subtilis | ||||||
![]() | YYCN protein | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / alpha and beta / fold / acetyltransferase / bacillus subtilis / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | ![]() acyltransferase activity, transferring groups other than amino-acyl groups / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Taneja, B. / Maar, S. / Shuvalova, L. / Collart, F.R. / Anderson, W. / Mondragon, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Structure of the bacillus subtilis YYCN protein: a putative N-acetyltransferase Authors: Taneja, B. / Maar, S. / Shuvalova, L. / Collart, F.R. / Anderson, W. / Mondragon, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.6 KB | Display | ![]() |
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PDB format | ![]() | 56.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.7 KB | Display | ![]() |
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Full document | ![]() | 442 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 18.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 20934.717 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Species: Bacillus subtilis / Strain: subsp. subtilis str. 168 / Gene: YYCN / Plasmid: pMCSG7 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.26 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 900mM Ammonium phosphate, 190mM Sodium chloride, 100mM Imidazole, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 22 ℃ / Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 20, 2003 | |||||||||
Radiation | Monochromator: Silicon mirrors / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||
Radiation wavelength |
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Reflection | Resolution: 2.2→26.08 Å / Num. all: 21340 / Num. obs: 21316 / % possible obs: 99.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.8 % / Rmerge(I) obs: 0.113 / Rsym value: 0.085 / Net I/σ(I): 6.8 | |||||||||
Reflection shell | Resolution: 2.2→2.32 Å / Redundancy: 3.5 % / Rmerge(I) obs: 0.318 / Mean I/σ(I) obs: 18.8 / Rsym value: 0.238 / % possible all: 99.3 | |||||||||
Reflection | *PLUS Highest resolution: 2.2 Å / Num. obs: 21192 / Rmerge(I) obs: 0.085 | |||||||||
Reflection shell | *PLUS Highest resolution: 2.2 Å / % possible obs: 99.3 % / Rmerge(I) obs: 0.238 / Mean I/σ(I) obs: 2.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.173 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→26.08 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.318 Å / Total num. of bins used: 10
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rfree: 0.259 / Rfactor Rwork: 0.233 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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