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Open data
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Basic information
Entry | Database: PDB / ID: 1tf4 | ||||||
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Title | ENDO/EXOCELLULASE FROM THERMOMONOSPORA | ||||||
![]() | T. FUSCA ENDO/EXO-CELLULASE E4 CATALYTIC DOMAIN AND CELLULOSE-BINDING DOMAIN | ||||||
![]() | GLYCOSYL HYDROLASE / CELLULOSE DEGRADATION | ||||||
Function / homology | ![]() cellulose binding / cellulase / cellulase activity / cellulose catabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sakon, J. / Wilson, D.B. / Karplus, P.A. | ||||||
![]() | ![]() Title: Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Authors: Sakon, J. / Irwin, D. / Wilson, D.B. / Karplus, P.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 277.3 KB | Display | ![]() |
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PDB format | ![]() | 230.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435 KB | Display | ![]() |
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Full document | ![]() | 443.5 KB | Display | |
Data in XML | ![]() | 59.1 KB | Display | |
Data in CIF | ![]() | 91.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.272878, 0.698058, 0.662007), Vector: |
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Components
#1: Protein | Mass: 67216.750 Da / Num. of mol.: 2 / Fragment: CATALYTIC DOMAIN AND CELLULOSE-BINDING DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene (production host): BAMH1-PST1 FRAGMENT OF T. FUSCA GENOMIC DNA CARRYING NATIVE E4 GENE Production host: ![]() #2: Chemical | ChemComp-CA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 52 % | ||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / pH: 5.8 Details: 90MG/ML E4-68 26% PEG8000 0.7M LICL 0.1M NA CITRATE, PH5.8 4 DEGREE, temperature 277K | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Jun 1, 1996 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→8 Å / Num. obs: 118700 / % possible obs: 94 % / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Rmerge(I) obs: 0.092 / Rsym value: 0.096 / Net I/σ(I): 11 |
Reflection shell | Resolution: 1.86→1.9 Å / Redundancy: 1.7 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 2.2 / Rsym value: 0.44 / % possible all: 73 |
Reflection shell | *PLUS % possible obs: 73 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 23 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINED | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.86→1.94 Å / Total num. of bins used: 10 /
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_improper_angle_deg / Dev ideal: 1.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Rfactor obs: 0.36 |