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Yorodumi- PDB-1tag: STRUCTURAL DETERMINANTS FOR ACTIVATION OF THE ALPHA-SUBUNIT OF A ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tag | ||||||
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Title | STRUCTURAL DETERMINANTS FOR ACTIVATION OF THE ALPHA-SUBUNIT OF A HETEROTRIMERIC G PROTEIN | ||||||
Components | TRANSDUCIN-ALPHA | ||||||
Keywords | GTP-BINDING PROTEIN / G-PROTEIN / GTPASE / TRANSDUCIN | ||||||
Function / homology | Function and homology information negative regulation of cyclic-nucleotide phosphodiesterase activity / G protein-coupled receptor complex / Inactivation, recovery and regulation of the phototransduction cascade / Activation of the phototransduction cascade / acyl binding / photoreceptor outer segment membrane / G alpha (i) signalling events / response to light stimulus / phototransduction / visual perception ...negative regulation of cyclic-nucleotide phosphodiesterase activity / G protein-coupled receptor complex / Inactivation, recovery and regulation of the phototransduction cascade / Activation of the phototransduction cascade / acyl binding / photoreceptor outer segment membrane / G alpha (i) signalling events / response to light stimulus / phototransduction / visual perception / photoreceptor inner segment / G protein-coupled receptor binding / G-protein beta/gamma-subunit complex binding / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / photoreceptor disc membrane / GDP binding / heterotrimeric G-protein complex / GTPase activity / GTP binding / protein kinase binding / metal ion binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | ||||||
Authors | Lambright, D.G. / Noel, J.P. / Hamm, H.E. / Sigler, P.B. | ||||||
Citation | Journal: Nature / Year: 1994 Title: Structural determinants for activation of the alpha-subunit of a heterotrimeric G protein. Authors: Lambright, D.G. / Noel, J.P. / Hamm, H.E. / Sigler, P.B. #1: Journal: Nature / Year: 1993 Title: The 2.2 Angstroms Crystal Structure of Transducin-Alpha Complexed with GTP-Gamma-S Authors: Noel, J.P. / Hamm, H.E. / Sigler, P.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tag.cif.gz | 84.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tag.ent.gz | 62.2 KB | Display | PDB format |
PDBx/mmJSON format | 1tag.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ta/1tag ftp://data.pdbj.org/pub/pdb/validation_reports/ta/1tag | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 37141.465 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Tissue: RETINA CELL: / Organ: EYE RETINA / References: UniProt: P04695 |
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#2: Chemical | ChemComp-MG / |
#3: Chemical | ChemComp-GDP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.04 Å3/Da / Density % sol: 59.54 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop / Details: using macroseeding | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 41071 / % possible obs: 98.3 % / Observed criterion σ(I): 3 |
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Reflection | *PLUS Highest resolution: 1.8 Å / Rmerge(I) obs: 0.124 |
-Processing
Software |
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Refinement | Resolution: 1.8→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 20.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.187 / Rfactor Rfree: 0.211 / Rfactor Rwork: 0.187 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 1.46 |