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Yorodumi- PDB-1sph: REFINED STRUCTURES OF THE ACTIVE S83C AND IMPAIRED S46D HPRS: EVI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sph | ||||||
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Title | REFINED STRUCTURES OF THE ACTIVE S83C AND IMPAIRED S46D HPRS: EVIDENCE THAT PHOSPHORYLATION DOES NOT REQUIRE A BACKBONE CONFORMATIONAL TRANSITION | ||||||
Components | HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR | ||||||
Keywords | PHOSPHOTRANSFERASE | ||||||
Function / homology | Function and homology information regulation of carbohydrate utilization / phosphoenolpyruvate-dependent sugar phosphotransferase system / cytoplasm Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | ||||||
Authors | Liao, D.-I. / Herzberg, O. | ||||||
Citation | Journal: Structure / Year: 1994 Title: Refined structures of the active Ser83-->Cys and impaired Ser46-->Asp histidine-containing phosphocarrier proteins. Authors: Liao, D.I. / Herzberg, O. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1992 Title: Structure of the Histidine-Containing Phosphocarrier Protein Hpr from Bacillus Subtilis at 2.0-Angstroms Resolution Authors: Herzberg, O. / Reddy, P. / Sutrina, S. / Saier Junior, M.H. / Reizer, J. / Kapadia, G. #2: Journal: J.Mol.Biol. / Year: 1990 Title: Crystallization of the Bacillus Subtilis Histidine-Containing Phosphocarrier Protein Hpr and Some of its Site-Directed Mutants Authors: Kapadia, G. / Reizer, J. / Sutrina, S. / Saier Junior, M.H. / Reddy, P. / Herzberg, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sph.cif.gz | 45.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sph.ent.gz | 32.4 KB | Display | PDB format |
PDBx/mmJSON format | 1sph.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sp/1sph ftp://data.pdbj.org/pub/pdb/validation_reports/sp/1sph | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.67413, -0.02956, -0.73802), Vector: Details | MTRIX THE TRANSFORMATIONS PRESENTED ON MTRIX RECORDS BELOW DESCRIBE NON-CRYSTALLOGRAPHIC RELATIONSHIPS AMONG THE VARIOUS DOMAINS IN THIS ENTRY. APPLYING THE APPROPRIATE MTRIX TRANSFORMATION TO CHAIN A WILL YIELD APPROXIMATE COORDINATES FOR CHAIN B. APPLIED TO TRANSFORMED TO MTRIX CHAIN RESIDUES CHAIN RESIDUES RMSD M1 A 2 - A 88 B 2 - B 88 0.29 | |
-Components
#1: Protein | Mass: 9226.345 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: PTSH / Plasmid: PRE GENE: PTSH / References: UniProt: P08877 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.55 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Kapadia, G., (1990) J.Mol.Biol., 212, 1. / PH range low: 5.4 / PH range high: 5.2 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 9111 / % possible obs: 92 % / Observed criterion σ(I): 0 |
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Reflection | *PLUS |
-Processing
Software |
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Refinement | Resolution: 2→10 Å / σ(F): 2 /
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Displacement parameters | Biso mean: 21.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.143 / Rfactor Rwork: 0.143 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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