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Open data
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Basic information
Entry | Database: PDB / ID: 1skz | ||||||
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Title | PROTEASE INHIBITOR | ||||||
![]() | ANTISTASIN | ||||||
![]() | SERINE PROTEASE INHIBITOR / ANTISTASIN / FACTOR XA INHIBITOR / THROMBOSIS | ||||||
Function / homology | ![]() negative regulation of coagulation / serine-type endopeptidase inhibitor activity / blood coagulation / heparin binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Krengel, U. / Dijkstra, B.W. | ||||||
![]() | ![]() Title: X-ray structure of antistasin at 1.9 A resolution and its modelled complex with blood coagulation factor Xa. Authors: Lapatto, R. / Krengel, U. / Schreuder, H.A. / Arkema, A. / de Boer, B. / Kalk, K.H. / Hol, W.G. / Grootenhuis, P.D. / Mulders, J.W. / Dijkema, R. / Theunissen, H.J. / Dijkstra, B.W. #1: ![]() Title: Refined X-Ray Structure of Antistasin at 1.9 A Resolution Authors: Krengel, U. / Lapatto, R. / Dijkstra, B.W. #2: ![]() Title: Crystallization and Preliminary Crystallographic Analysis of Antistasin, a Leech-Derived Inhibitor of Blood Coagulation Factor Xa Authors: Schreuder, H. / Arkema, A. / De Boer, B. / Kalk, K. / Dijkema, R. / Mulders, J. / Theunissen, H. / Hol, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 30.5 KB | Display | ![]() |
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PDB format | ![]() | 23.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 410 KB | Display | ![]() |
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Full document | ![]() | 411 KB | Display | |
Data in XML | ![]() | 7.1 KB | Display | |
Data in CIF | ![]() | 9.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13375.696 Da / Num. of mol.: 1 / Mutation: Q1E, G2D, M35V Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Organ: BLOOD / Production host: ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 41.8 % Description: THE FINAL HIGH RESOLUTION DATA SET WAS OBTAINED BY SCALING 3 PARTIAL DATA SETS, ONE FROM DESY (TO 1.9 A) AND TWO DATA SETS COLLECTED IN HOUSE (BOTH TO 2.5 A). | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 6 Details: EQUILIBRATION AGAINST 100 MM NA CITRATE (PH6.0), 1MM PMSF, 10 MM NA AZIDE, 31% (W/V) AMMONIUM SULFATE, 2.75 M NA CHLORIDE IN HANGING DROPS. FOR CRYO EXPERIMENTS TRANSFER TO 100 MM NA CITRATE ...Details: EQUILIBRATION AGAINST 100 MM NA CITRATE (PH6.0), 1MM PMSF, 10 MM NA AZIDE, 31% (W/V) AMMONIUM SULFATE, 2.75 M NA CHLORIDE IN HANGING DROPS. FOR CRYO EXPERIMENTS TRANSFER TO 100 MM NA CITRATE (PH6.0), 30% (W/V) AMMONIUM SULFATE, 2.2 M NA CHLORIDE, 20% GLYCEROL, AFTER PREEQUILIBRATION AGAINST THIS SOLUTION., vapor diffusion - hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: Schreuder, H., (1993) J.Mol.Biol., 231, 1137. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→9.9 Å / Num. obs: 10300 / % possible obs: 95 % / Observed criterion σ(I): 0 / Redundancy: 9.7 % / Rmerge(I) obs: 0.08 / Rsym value: 0.38 / Net I/σ(I): 173 |
Reflection shell | Resolution: 1.9→2 Å / Redundancy: 8.2 % / Rmerge(I) obs: 0.071 / Mean I/σ(I) obs: 110 / Rsym value: 0.071 / % possible all: 92 |
Reflection | *PLUS Num. measured all: 100326 / Rmerge(I) obs: 0.08 |
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Processing
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Refinement | Method to determine structure: ![]() Details: DURING THE REFINEMENT, 10% OF THE REFLECTIONS WERE SET ASIDE TO CALCULATE THE FREE R-FACTOR. THE LAST ROUND OF REFINEMENT, WHICH RESULTED IN AN R-FACTOR 0.215 AND A FREE R-FACTOR OF 0.274, ...Details: DURING THE REFINEMENT, 10% OF THE REFLECTIONS WERE SET ASIDE TO CALCULATE THE FREE R-FACTOR. THE LAST ROUND OF REFINEMENT, WHICH RESULTED IN AN R-FACTOR 0.215 AND A FREE R-FACTOR OF 0.274, WAS THEN REPEATED WITH ALL THE REFLECTIONS FOR THE FINAL RESULTS.
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Displacement parameters | Biso mean: 26 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.28 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→5 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.98 Å / Total num. of bins used: 8
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Xplor file |
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