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- PDB-1sky: CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1sky | ||||||
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Title | CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3 | ||||||
![]() | (F1-ATPASE) x 2 | ||||||
![]() | ATP SYNTHASE / F1FO ATP SYNTHASE / F1-ATPASE / ALPHA3BETA3 SUBCOMPLEX OF F1-ATPASE / HYDROLASE | ||||||
Function / homology | ![]() : / H+-transporting two-sector ATPase / proton-transporting ATPase activity, rotational mechanism / proton-transporting ATP synthase activity, rotational mechanism / ADP binding / ATP hydrolysis activity / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Shirakihara, Y. / Leslie, A.G.W. / Abrahams, J.P. / Walker, J.E. / Ueda, T. / Sekimoto, Y. / Kambara, M. / Saika, K. / Kagawa, Y. / Yoshida, M. | ||||||
![]() | ![]() Title: The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer. Authors: Shirakihara, Y. / Leslie, A.G. / Abrahams, J.P. / Walker, J.E. / Ueda, T. / Sekimoto, Y. / Kambara, M. / Saika, K. / Kagawa, Y. / Yoshida, M. #1: ![]() Title: X-Ray Crystal Analysis of Alpha3Beta3 Complex of F1-ATPase from a Thermophilic Bacterium Ps3 Authors: Shirakihara, Y. / Ueda, T. / Sekimoto, Y. / Yoshida, M. / Saika, K. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 190.8 KB | Display | ![]() |
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PDB format | ![]() | 151.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.7 KB | Display | ![]() |
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Full document | ![]() | 483.8 KB | Display | |
Data in XML | ![]() | 37.8 KB | Display | |
Data in CIF | ![]() | 51 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54648.328 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PKK ALPHA FOR ALPHA SUBUNIT, PUC118BETA FOR BETA SUBUNIT Production host: ![]() ![]() | ||
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#2: Protein | Mass: 51998.082 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Plasmid: PKK ALPHA FOR ALPHA SUBUNIT, PUC118BETA FOR BETA SUBUNIT Production host: ![]() ![]() | ||
#3: Chemical | Compound details | ALPHA3 BETA3 SUBCOMPLEX OF F1-ATPASE COMPRISES THREE ALPHA AND THREE BETA SUBUNITS. BECAUSE THE ...ALPHA3 BETA3 SUBCOMPLEX | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61 % Description: J.P. ABRAHAMS, A.G.W. LESLIE, R. LUTTER, J.E. WALKER STRUCTURE AT 2.8A RESOLUTION OF F1-ATPASE FROM BOVINE HEART MITOCHONDRIA, NATURE 370,621-628, (1994) | ||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 8 Details: HANGING DROP WITH 9-11% PEG20000, 0.12 M SODIUM SULFATE AT PH8.0, EQUILIBRATED AGAINST PROTEIN FREE RESERVOIR WITH HIGHER PEG SOLUTION BY 2%. KEPT AT 15DEG., vapor diffusion - hanging drop, temperature 288K | ||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 15 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Detector: IMAGE PLATE / Date: May 24, 1994 / Details: MIRROR + MONOCHROMATOR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. obs: 109106 / % possible obs: 99 % / Redundancy: 4.6 % / Biso Wilson estimate: 83 Å2 / Rmerge(I) obs: 0.075 / Net I/σ(I): 8.9 |
Reflection shell | Resolution: 3.2→3.37 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.44 / Mean I/σ(I) obs: 1.5 / % possible all: 98.1 |
Reflection | *PLUS Num. obs: 22429 / Num. measured all: 109106 |
Reflection shell | *PLUS % possible obs: 98.1 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: MITOCHODRIAL F1-ATPASE Resolution: 3.2→6 Å
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Displacement parameters | Biso mean: 68 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.2→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.32 Å / Total num. of bins used: 8
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rwork: 0.37 |